川芎嗪对N-甲基-N-亚硝基脲致光感受器细胞损伤的保护作用及其机制  

Protective effects of ligustrazine against photoreceptor cell injury induced by N-methyl-N-nitrosourea and its mechanism

作　　者：杨锦南[1] 许建功[2] 陈金卯[3] 林少春[3] 罗琳[3] 胡世兴[4] 

机构地区：[1]新乡医学院药学院,河南新乡453003 [2]新乡医学院第三附属医院,河南新乡453003 [3]中山大学中山眼科中心,广东广州510060 [4]中国中医研究院眼科医院,北京100040

出　　处：《药学学报》2005年第8期690-694,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30300467).

摘　　要：目的探讨川芎嗪对N-甲基-N-亚硝基脲(NmethylNnitrosourea,MNU)致大鼠视网膜损伤的作用。方法大鼠生后47d,腹腔注射不同剂量的川芎嗪注射液;50d注射MNU60mg·kg-1。测量视网膜总厚度;TUNEL和RTPCR法分别检测细胞凋亡、cjun和cfos的表达。结果川芎嗪注射液可剂量依赖性地增加周边视网膜总厚度和降低凋亡指数,并抑制MNU诱导的即早基因表达。结论川芎嗪通过下调基因c-jun和c-fos的表达,抑制光感受器细胞凋亡,从而部分保护MNU引起的视网膜损伤。Aim To study the protective effect of ligustrazine against photoreceptor cell injury induced by N-methyl-N-nitrosourea （MNU） in Sprague-Dawley （SD） rats. Methods Ligustrazine injections of different doses were injected intraperitoneally into 47-day female SD rats once a day and a single intraperitoneal injection of MNU 60 mg· kg^-1 was given to 50-day rats. At different intervals after MNU treatment,the animals were sacrificed. The apoptotic index of photoreceptor ceils was calculated by TUNEL labeling at 24 h following MNU treatment; peripheral retinal damage was evaluated based on retinal thickness at the d 7 after MNU treatment, and the expression of c-jun and c-fos genes was detected by RT-PCR technique. Results Ligustrazine injection could remarkably increase total thickness of peripheral retina and decrease apoptotic index of photoreceptor cells induced by MNU in a dose-dependent manner. Compared with MNU-treated rats, the gene expression of c-jun and c-fos was time-dependently down-regulated in ligustrazine-treated group. Conclusion Ligustrazine injection partially protects against MNU-induced retinal damage by down-modulating the expression of c-jun and c-fos genes to inhibit apoptosis of photoreceptor cells.

关 键 词：川芎嗪 N-甲基-N-亚硝基脲 光感受器细胞 细胞凋亡 

分 类 号：R282.71[医药卫生—中药学] R965.1[医药卫生—中医学]