长时间电针时大鼠脑内八肽胆囊收缩素的生成和释放加速  

ACCELERATED RELEASE AND PRODUCTION OF CCK-8 IN CENTRAL NERVOUS SYSTEM OF RATS DURING PROLONGED ELECTROACUPUNCTURE STIMULATION

作　　者：孙宇华[1] 周岩[1] 张志文[1] 韩济生[1] 

机构地区：[1]北京医科大学神经科学研究中心

出　　处：《神经科学》1995年第2期83-88,共6页Chinese Journal of Neuroscience

基　　金：国家自然科学基金;MDA grant(DA03983)资助

摘　　要：本文用大鼠进行实验，观察了长时间电针刺激时脑脊液和中脑导水管周围灰质内ＣＣＲ－８样免疫活性物质（ＣＣＫ－８－ｉｒ）含量，并用Ｎｏｒｔｈｅｒｎ印迹法测定脑内ＣＣＫｍＲＮＡ含量的变化。结果表明，电针１ｈ后大鼠脑脊液中ＣＣＫ－８－ｉｒ的浓度增高，２ｈ后中脑导水管周围灰质的含量也明显升高，８ｈ后大鼠脑内ＣＣＫ－８ｍＲＮＡ含量显著增加。表明长时间电针加速脑内ＣＣＫ—８的生成和释放，最终加速了ＣＣＫ基因表达，这可能是ＣＣＫ－８参与电针耐受的机理之一。Experiments were performed in rats in order to observe the effects of prolonged electroacupuncture (EA) stimulation on the content of immunoreactive (ir) cholecystokinin octapeptide (CCK-8) in CSF and in the tissue of periaqueductal gray (PAG).Changes in the brain content of mRNA coding for preprocholecystokinin was also determined by the Northern blot technique. The results showed a significant increase in the content of ir -CCK-8 both in the spinal perfusates and in the tissue of PAG at the end of 1h and 2h of continuous EA stimulation, respectively.The brain content of CCK mRNA did not show any significant increase until 8h of continuous stimulation. It is concluded that prolonged EA stimulation elicited an accelerated release of CCK-8 and the processing of CCK -8 from its precursors, followed by a delayed response of CCK gene expression. This may constitute one of the mechanisms for the development of EA tolerance.

关 键 词：电针耐受 八肽胆囊收缩素 基因表达 分子杂交 

分 类 号：R338.3[医药卫生—人体生理学]