地高辛素标记人神经生长因子DNA探针的制备及应用  

PURIFICATION AND ITS APPLICATION OF THE DIGOXIGENIN -LABELED HUMAN-β NGF DNA PROBE

作　　者：曹铮[1] 陆璐 丁斐[1] 顾静 刘飞 殷冬梅 顾晓松[1] 余云开[1] 汪家政[1] 

机构地区：[1]江苏南通医学院分子生物学研究室,北京军事医学科学院基础医学研究所

出　　处：《神经科学》1995年第3期141-144,140,共4页Chinese Journal of Neuroscience

摘　　要：地高辛素标记人神经生长因子ＤＮＡ探针的制备及应用曹铮，陆璐，丁斐，顾静，刘飞，殷冬梅，顾晓松江苏南通医学院分子生物学研究室南通２２６００１余云开，汪家政北京军事医学科学院基础医学研究所北京１００８５０ＰＵＲＩＦＩＣＡＴＩＯＮＡＮＤＩＴＳＡＰＰＬＩＣＡ...Recombinant human-βNGF DNA/pUC 19 was digested with Hind Ⅲ and Bam HI and h-βNGF DNA fragments were further purified by low-melting agarose electrophoresis. H-βNGF DNA were digoxigenin labeled with random priming. It could detect even 0.1 pg/μl h-βNGF DNA/pUC 19 in dot hybridization.In situ hybridization of the submandibular glands also showed the high specificity and sensitivity of this probe in measuring β-NGF mRNA.

关 键 词：神经生长因子 DNA探针 地高辛 制备 应用 

分 类 号：Q422[生物学—神经生物学] R33－33[生物学—生理学]