宫内乙醇暴露对胎鼠大脑线粒体蛋白质组的影响  

In utero ethanol exposure alters the mitochondrial protein expression of fetal mouse cerebrum

作　　者：许雅君[1] 张国庆[2] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100083 [2]中国人民解放军总医院医学实验测试中心

出　　处：《北京大学学报（医学版）》2005年第4期346-350,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30271364)资助~~

摘　　要：目的:探讨宫内乙醇暴露对胚胎大脑线粒体蛋白组的影响.方法:CD-1孕鼠随机分成对照组和5.0g/kg乙醇染毒组,染毒期为孕6～15 d,孕18天剖腹取胎,选取没有明显外观畸形的胎鼠进行实验.提取胎鼠大脑线粒体进行全蛋白双向凝胶电泳分析,并用质谱鉴定差异表达的蛋白点,同时进行线粒体呼吸酶活性和大脑细胞ATP含量检测.结果:宫内乙醇暴露组胎鼠大脑线粒体蛋白表达发生改变.呼吸酶复合物Ⅳ(相当于对照组的72.3%±4.6%)和三磷酸腺苷(ATP)合成酶活性(相当于对照组的80.3%±5.1%)均较对照组降低,大脑细胞内ATP含量下降(相当于对照组的67.9%±3.9%).结论:宫内乙醇暴露能够影响胚胎大脑线粒体蛋白表达,即使没有出现明显的形态畸形,胚胎大脑细胞能量状态已经受到影响,可能与孕期饮酒导致的后代神经行为障碍有关.Objective：To probe the effect of prenatal ethanol exposure on the mitochondrial protein expression in fetal mouse cerebrum. Methods：Pregnant CD-1 mice were given 5.0 g/kg ethanol intragastrically from pregnant days （PD） 6-15. Fetal cerebral mitochondria were isolated on PD 18. The overall mitochondrial protein was applied to two-dimensional gel electrophoresis. Differentially expressed protein spots were cut off and identified by matrix-assisted laser desorption ionization time-of-flight （ MALDITOF） mass spectrometry. Activities of respiratory chain complex Ⅳ （72. 3 % ± 4. 6% of control） and ATP synthase, and ATP content in the fetal cerebral cells were measured. Results：Expressions of some constructional and functional proteins were altered by in utero ethanol exposure. Activities of respiratory chain complex Ⅳ and ATP synthase （80. 3% ± 5.1% of control） were both decreased. ATP content （67.9% ±3.9% of control） in the cerebral cells was reduced in the in utero ethanol exposure fetuses. Conclusion：In utero ethanol exposure affects the mitochondrial protein expression of fetal mouse cerebrum, even in the tissue without obvious morphological malformations, which may be a possible mechanism of behavioral defects found in children with prenatal ethanol exposure.

关 键 词：乙醇 蛋白质组 线粒体 子宫 小鼠 

分 类 号：R994.4[医药卫生—毒理学]