双硒唑烷-1的动物体内抗肿瘤作用  

The antitumor activity of Shuang-Xi-Zuo-Wan-1 in C57/BL mice

作　　者：王怡瑞[1,2] 肖军军[2] 董晓敏[2] 孟书聪[2] 邓声菊[1] 况斌[1] 严俊[1] 赵芳[1] 曾慧慧[1] 

机构地区：[1]北京大学药学院,北京100083 [2]北京大学基础医学院细胞生物学系

出　　处：《北京大学学报（医学版）》2005年第4期421-424,共4页Journal of Peking University:Health Sciences

摘　　要：目的:检测新型有机硒化合物双硒唑烷-1(Eb)的动物体内抗肿瘤作用。方法:建立Lewis肺癌(lewislungcancer,LLC)皮下移植瘤C57/BL鼠动物模型,选取25.0mg/kg和12.5mg/kg两个剂量的Eb作为实验组,以2.0mg/kg剂量的顺铂(DDP)作为阳性对照,以溶剂5g/L羧甲基纤维素钠溶液为阴性对照,于接种肿瘤后第2天开始向C57/BL鼠腹腔连续注射药物7d,探讨Eb对荷瘤鼠的存活期、肿瘤的生长速度、浸润级别、细胞形态、细胞周期和细胞凋亡的影响。结果:Eb能够明显抑制肿瘤生长和侵袭(高剂量Eb的肿瘤抑制率为80.31%),延长荷瘤鼠的存活期;形态学观察发现,给予Eb后肿瘤细胞核浓缩深染,分裂相细胞减少;流式细胞仪检测结果表明,Eb能够促进肿瘤细胞的凋亡。结论:新型有机硒化合物Eb在C57/BL小鼠体内能够明显抑制LLC的生长和侵袭,促进肿瘤细胞的凋亡,具有较强的抗肿瘤活性。Objective ： To detect the antitumor activity of Shuang-Xi-Zuo-Wan-1 （ Eb ）, a novel organoselenium compound, in C57/BL mice transplanted with Lewis lung cancer（LLC）. Methods： The LLC transplanted C57/BL mice model was established, and the mice were randomly divided into four groups, including high dose Eb group （25.0 mg/kg） ,low dose Eb group （12.5 mg/kg）, positive control group （DDP,2.0 mg/kg） and negative control group（solvent）. Each group had twelve mice. Intraperitoneal injections （ip.） of four pharmaceuticals were performed once a day through the abdominal wall separately, from the second to the eighth days after cancer was transplanted. On the eleventh day, six mice of each group were killed and the influences of Eb on growth speed ,size, weight, invasion, inhibitory rate, proliferation index and apoptosis rate of LLC were observed and calculated. The remaining mice were fed till all of them died naturely and the average survival time of each group was calculated. Results：Eb could inhibit the growth and infiltration of LLC （the cancer inhibitory rate of high does Eb was 80.31% ） obviously and prolong the average survival time of these with mice cancer. After being given Eb,the nuclear of the cancer cell concentrated and the fission phase cells reduced. In addition, the number of apoptosis cancer cells increased. Conclusion：The novel organoselenium compound Eb has antitumo activity in vivo . It can inhibit the growth and infiltration of LLC in mice, and induce the apoptosis of cancer cells.

关 键 词：有机硒化合物 抗肿瘤药 顺铂 

分 类 号：R979.1[医药卫生—药品]