菲咯啉铜配合物模拟过氧化物酶检测过氧化氢  

Determination of Hydrogen Peroxide Using Phenanthroline Copper Chelate as Mimic of Peroxidase

作　　者：林弘[1] 李得加[1] 李希文[1] 邹国林[1] 

机构地区：[1]武汉大学生命科学学院

出　　处：《武汉大学学报（理学版）》2005年第4期497-500,共4页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(39770200)

摘　　要：以菲咯啉铜[Cu(OP)2]配合物模拟辣根过氧化物酶催化过氧化氢与邻苯二胺反应,研究了不同反应条件对催化活性的影响.据此,建立了以菲咯啉铜模拟酶体系检测过氧化氢的灵敏方法.测定所用的试剂不含辣根过氧化物酶,从而避免了生物酶在使用过程中因可能的失活给分析工作带来的困难.在最佳条件下,过氧化氢浓度在9.5×10-8～1.67×10-6 mol/L的范围内与体系吸光度成良好的线性关系.检测极限为8.0×10-8 mol/L.（phen）2 Cu was used as a mimetic enzyme for peroxidase to catalyze the oxidative reaction of o-phenylenediamine with hydrogen peroxide as an oxidant. The effects of different conditions on its catalytic activity were studied. A sensitive spectrophotoscopic method for determination of trace amount of hydrogen peroxide was proposed based on the catalytic effect of （phen）2 Cu on the reaction of o-phenylenediamine and hydrogen peroxide. Under the optimal conditions, hydrogen peroxide concentration has a good linear relationship with the system absorption in the range of 9.5× 10^-8 to 1.67× 10^-6 mol/L hydrogen peroxide, and tile determination limit was 8.0×10^-8 mol/L.

关 键 词：菲咯啉铜 模拟酶 过氧化氢 

分 类 号：Q55[生物学—生物化学]