大鼠INS-1细胞中IGFBP-2mRNA的表达调控  

Regulation of IGFBP-2 gene expression in rat insulin producing cell line INS-1

作　　者：张春举[1] 袁栎[1] 周锦勇[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学与分子生物学系,江苏南京210029

出　　处：《第四军医大学学报》2005年第16期1448-1450,共3页Journal of the Fourth Military Medical University

基　　金：江苏省自然科学基金(BK2001119)

摘　　要：目的:研究大鼠胰腺肿瘤β-细胞(INS-1)中,葡萄糖(Glucose)、胰岛素(Insulin)和生长激素(GH)对胰岛素样生长因子结合蛋白-2(IGFBP-2)基因的表达调控,探索IGFBP-2基因调节机制及其在维持正常胰腺细胞生理功能中的作用.方法:RNA印迹法(Northern blotting)检测INS-1细胞IGFBP-2 mRNA的表达,酶标法测定INS-1细胞胰岛素分泌量.结果:葡萄糖对IGFBP-2 mRNA调节作用不同;高浓度(10mmol/L)葡萄糖条件下,胰岛素分泌量增加,而IGFBP-2mRNA表达被抑制;低浓度(1～5.6 mmol/L)葡萄糖条件下,胰岛素分泌量无显著性变化,IGFBP-2 mRNA正常表达.INS-1细胞在不完全培养液中,加入GH培养,在不同时间间隔提取培养液进行IGFBP-2 mRNA检测,结果发现,培养16 h以上,IGFBP-2 mRNA表达开始显著下降.外源胰岛素抑制IGFBP-2 mRNA的表达.结论:葡萄糖和生长激素对INS-1细胞中IGFBP-2 mRNA表达的抑制作用是通过胰岛素调控的.AIM： To study the effects of glucose, insulin and growth hormone （GH） on expression of IGFBP-2 （insulin-like growth factor binding protein 2） mRNA in INS-1 cell line and to analyze the mechanism of IGFBP-2 regulation. METHODS： Northern blot analysis was performed for the expression of IGFBP-2 mRNA and enzyme-linked immunoadsorbent assay for the determination of insulin release in this cell-line. RESLUTS： Northern blot analysis showed that different glucose concentration could make a discordant effect on IGFBP-2 gene expression. The secretion of insulin increased and the expression of IGFBP-2 mRNA was suppressed when cells were kept at high glucose concentration. On the contrary, IGFBP-2 mRNA could be well detected in cells that were kept at low glucose while the insulin secreted normally. After 16h incubation, exogenous GH inhibited the expression of IGFBP-2 and at the same time the secretion of insulin increased. The expression of IGFBP-2 mRNA was inhibited by exogenous insulin at a low glucose concentration condition. CONCLUSION： The effects of glucose and GH on the expression of IGFBP-2 mRNA are inhibited by insulin.

关 键 词：胰岛素样生长因子结合蛋白质2 RNA 信使 胰岛素 葡萄糖 生长激素 INS-1细胞 

分 类 号：Q455[生物学—生理学]