从噬菌体表面展示环状7肽肽库中筛选葡萄球菌B型肠毒素抑制剂  

Screening inhibitor of Staphylococcal enterotoxin B from random circular 7-mer peptide library displayed on phage

作　　者：李韩平[1] 周宏[1] 郑玉玲[1] 邓小红[1] 马茹[1] 姜永强[1] 

机构地区：[1]军事医学科学院微生物与流行病研究所,北京100071

出　　处：《第四军医大学学报》2005年第16期1460-1463,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目(30171091);北京市自然科学基金资助项目(7022031);军事医学科学院青年创新基金(0108011)

摘　　要：目的:拟通过生物淘选从噬菌体表面展示环状7肽肽库中筛选出与葡萄球菌B型肠毒素(Staphylococcalenter-otoxinB,SEB)结合并能抑制该毒素毒性效应的特异性短肽.方法:采用Phage-ELISA来鉴定所得目的肽的亲和性;利用竞争ELISA研究合成肽与SEBmAb竞争结合SEB的情况;通过动物实验考察其抑制SEB的超抗原特性和肠毒活性情况.结果:筛选所得短肽在一定浓度范围内可以抑制SEB对鼠脾淋巴细胞的激活;合成肽与SEB质量比160∶1下,合成肽可较好地抑制SEB对乳猫的肠毒活性,并对SEB引起的小鼠致死具有明显保护作用.结论:得到了能与SEB特异结合并能抑制SEB超抗原特性和肠毒活性的短肽.AIM： To obtain the specific peptide which can bind with SEB and inhibit SEB＇s enterotoxin activity by panning from the random circular 7-mer polypeptide libraries. METHODS： The affinity of peptide and the competitive activity of the synthetic peptide with that of anti-SEB monoclonal antibody （McAb） were detected respectively by Phage-ELISA and competition ELISA. Animal experiment was performed to assess the suppressive effect of the screened peptide on the toxicity of SEB and its enterotoxin. RESULTS： The circular 7-mer peptide obtained by panning suppressed the multiplication of spleen lymphocytes in mice. At the ratio of 160：1 （SEB： synthetic 7-mers peptide）, the peptide protected the little cats from attack of enterotoxin and the survival rate of mice treated with the screened peptide was significantly higher compared with that of the control group. CONCLUSION： The obtained peptide specifically combines with SEB and suppresses its enterotoxin activity, which paves the way for the development of SEB inhibitors.

关 键 词：葡萄球菌B型肠毒素 肽库 筛选 抑制剂 

分 类 号：R392.11[医药卫生—免疫学]