Egr-1基因剔除对雨蛙素诱导小鼠胰腺炎的影响  

Knockout Egr-1 ameliorates cearulein-induced pancreatitis in mice

作　　者：段佑才[1] 姜泊[2] 智发朝[2] 宋于刚[2] 陈学清[2] 

机构地区：[1]广东省第二人民医院内科,广东广州510317 [2]广州南方医院消化内科,广东广州510515

出　　处：《第四军医大学学报》2005年第16期1481-1483,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30370648)

摘　　要：目的:探讨Egr-1在实验性胰腺炎发病中的作用.方法:利用Egr-1基因剔除小鼠,采用大剂量雨蛙素诱导的实验性胰腺炎模型,观察Egr-1基因剔除后,胰腺组织水肿,组织髓过氧化物酶(MPO)水平,血清淀粉酶水平,肺组织MPO水平,以及胰腺与肺组织学改变.结果:与野生型相比,Egr-1基因剔除小鼠胰腺组织水肿明显减轻[(166.0±13.5)%vs(132.3±17.7)%,P<0.05],但组织MPO水平与血清淀粉酶与野生型组间相比无明显改变;肺组织MPO水平与野生型组相比明显降低[(168.1±41.7)%vs(274.0±98.7)%,P<0.05].在组织学上,Egr-1基因剔除组胰腺和肺组织学明显改善,但胰腺腺泡仍有空泡变性及轻度肿胀.结论:Egr-1基因剔除可明显减轻实验性胰腺炎胰腺和肺组织的损伤.AIM： To study the role of Egr-1 in the initiation of acute pancreatitis. METHODS： Experimental pancreatitis was induced in the Egr-1 knockout mice with high-dose of cearulein. The pancreatitis indices such as water content of pancreas, serum amylase and myeloperoxidase （MPO） activities were measured. The histology of pancreas and lung was observed under microscopy. RESULTS： The water content of pancreata in Egr-1 knockout mice was significantly reduced [ （ 166.0 ± 13.5）% vs （ 132.3 ± 17. 7）%, P 〈0.05 ]. No significant difference of MPO levels in pancreata and serum amylases was found between the wildtype and Egr-1 knockout mice. However, the lung MPO levels in Egr-1 knockout mice were significantly reduced compared with those in wildtype mice [（168.1 ± 41.7）% vs （274.0 ± 98.7% ）, P〈0.05]. In Egr-1 knockout mice, the histological appearance in pancreas and lung improved, though some vacuoles in acinar cells and light edema in pancreas were observed in pancreas. CONCLUSION： The severity of pancreatitis and lung damage is ameliorated in Egr-1 knockout mice stimulated by high-dosage of cearulein, suggesting that Egr-1 is one of the important factors in the initiation of acute pancreatitis.

关 键 词：急性病 胰腺炎 早期生长反应因子-1 小鼠 基因敲除 

分 类 号：R33-33[医药卫生—人体生理学]