子宫内膜异位症在位和异位内膜水通道蛋白的表达  

Expression of aquaporins in eutopic and ectopic endometrium of patients with endometriosis

作　　者：郭晓燕[1] 吴瑞瑾[1] 林俊[1] 

机构地区：[1]浙江大学医学院附属妇产科医院中心实验室,浙江杭州310006

出　　处：《吉林大学学报（医学版）》2011年第6期1015-1018,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30772326)

摘　　要：目的:研究水通道蛋白2(AQP2)和水通道蛋白8(AQP8)在子宫内膜异位症(EMs)患者在位和异位内膜中的表达,阐明AQP2和AQP8与EMs发生和发展的关系。方法:选取本院住院患者53例,其中38例为EMs患者,每例均取其在位内膜和异位内膜,分别为EMs在位内膜组(Eu-Em组)和EMs异位内膜组(Ec-Em组);另外15例对照组患者正常子宫内膜为对照组(Co-Em组)。通过Real time RT-PCR和Western blotting分别检测Co-Em组、Eu-Em组和Ec-Em组子宫内膜中AQP2和AQP8的mRNA水平和蛋白表达水平。结果:与Co-Em组比较,Eu-Em组AQP2和AQP8 mRNA水平差异无统计学意义(P>0.05);Ec-Em组的AQP2 mRNA水平显著高于其他2组(P<0.05),Ec-Em组的AQP8 mRNA水平显著低于其他2组(P<0.05)。与Co-Em组比较,Eu-Em组AQP2和AQP8蛋白表达水平差异无统计学意义(P>0.05),而Ec-Em组AQP2和AQP8蛋白表达水平与其他2组比较均明显下降(P<0.05)。结论:水通道蛋白(AQP2和AQP8)在EMs患者在位和异位内膜中表达不同,AQPs可能是EMs病理发生和发展的一个重要环节。Objective To investigate the expressions of aquaporin 2(AQP2) and aquaprin 8(AQP8) in eutopic and ectopic endometrium of patients with endometriosis(EMs)and to elucidate possible impact of AQP2 and AQP8 on EMs.Methods 53 patients in our hospital were selected,among them 37 cases were EMs patients,they were divided into eutopic endometrium(Eu-Em) group and ectopic endometrium(Ec-En) group;15 healthy subjects were used as control(Co-Em) group.The AQP2 and AQP8 mRNA and protein expressions in endometrium tissues in Eu-Em group,Ec-Em group and Co-Em group were detected with real-time reverse-transcriptase polymerase chain reaction and Western blotting.Results Compared with Co-Em group,the AQP2 and AQP8 mRNA levels in Eu-Em group had no significant difference(P<0.05);the AQP2 mRNA level in Ec-Em group was significantly high than those in Co-Em and Eu-Em(P<0.05),the AQP8 mRNA level in Ec-Em group was significantly lower than those in Co-Em and Eu-Em group(P<0.05).Compared with Co-Em group,the AQP2 and AQP8 protein expressions in Eu-Em group had no significant difference(P>0.05),but the AQP2 and AQP8 protein expression levels in Ec-Em group were significantly lower than those in Co-Em and Eu-Em groups(P<0.05).Conclusion AQP2 and AQP8 may play an important role in occurence and development of endometriosis.

关 键 词：水通道蛋白 子宫内膜异位症 细胞迁徙 

分 类 号：R711[医药卫生—妇产科学]