黄芪对支气管哮喘大鼠支气管肺泡灌洗液及血清中NO、IL-5和炎症细胞的影响  

Effect of mongolian milkvetch root on NO,IL-5,inflammatory cells in BALF and serum of bronchial asthmatic rats

作　　者：张雨[1] 韦倩[2] 伊鹏霏[3] 

机构地区：[1]吉林大学和平校区门诊部,吉林长春130062 [2]长春中医药大学附属医院心病科,吉林长春130021 [3]吉林大学畜牧兽医学院兽医临床学教研室,吉林长春130062

出　　处：《吉林大学学报（医学版）》2011年第6期1033-1036,共4页Journal of Jilin University：Medicine Edition

基　　金："十一五"国家科技支撑计划项目资助课题(2008BADB4B04-4)

摘　　要：目的:探讨黄芪对支气管哮喘模型大鼠血清及支气管肺泡灌洗液(BALF)中一氧化氮(NO)、白细胞介素5(IL-5)和炎症细胞的影响,阐明黄芪对哮喘的治疗效果。方法:60只Wistar大鼠随机分为正常对照组、模型组、激素干预组及黄芪干预低、中、高剂量组,每组10只,除正常对照组外以卵白蛋白(OVA)致敏并吸入激发法制备大鼠哮喘模型,干预组分别于实验21d给予黄芪2mL(0.3g.100g-1体质量)灌胃或普米克令舒2mL雾化吸入,每天1次,连续14d,正常对照组与模型组大鼠给予等量生理盐水灌胃,每天1次,连续14d。观察大鼠行为学变化,同时检测各组大鼠血清及BALF中NO、IL-5和炎症细胞的变化。结果:根据行为学观察大鼠哮喘造模成功。经治疗,大鼠行为状态转好,但未达到正常状态;黄芪干预组大鼠BALF和血清中NO和IL-5的含量较哮喘模型大鼠明显减少(P<0.01),BALF中炎症细胞总数、嗜酸性粒细胞百分比明显减少(P<0.01)。结论:黄芪能够抑制哮喘模型大鼠BALF中IL-5和NO的增多,同时能够明显减少气道炎症细胞和嗜酸性细胞的活化和浸润,从而减轻呼吸道炎症,减轻哮喘症状或避免哮喘发作,达到防治哮喘的目的。Objective To observe the effect of mongolian milkvetch root on nitric oxide(NO),interleukin 5(IL-5),inflammatory cells in bronchoalveolar lavage fluid(BALF) and serum of bronchial asthmatic rats and clarify the efficacy of mongolian milkvetch root in treatment of asthma.Methods 60 Wistar rats were randomly divided into normal group,model group,budesonide suspension for inhalation intervention group and low,middle,and high dose mongolian milkvetch root intervention groups,10 rats in each group.The rat bronchial asthma models were established by egg protein(OVA) sensibilization and long-term inhalation provocation.The rats in mongolian milkvetch root intervention groups were lavaged with mongolian milkvetch root 2 mL(0.3 g·100g-1),and the rats in budesonide suspension for inhalation intervention group were inhalated with budesonide suspension 2 mL at 21 d after expreriment;the rats in normal group and model group were lavaged with normal saline orally,once each day for 14 d.The behavior of rats was observed and the changes of NO,IL-5,inflammatory cells in BALF and serum of bronchial asthmatic rats in each group were detected.Results The model of asthma was set up successfully by observing the behavior of rats,then the behavior of the rats became better after treatment,but did not reach the normal state.The NO and IL-5 levels in BALF and serum in mongolian milkvetch root intervention groups were significantly reduced compared with model group(P<0.01),and the percentages of the total inflammatory cells and eosinophils in BALF were significantly reduced(P<0.01).Conclusion Mongolian milkvetch root could inhibit the increasing of IL-5 and NO in BALF in asthmatic models,and could significantly reduce activation and infiltration of airway inflammatory cells and eosinophils,thus reduce airway inflammation and asthma symptoms or avoid the onset of asthma to achieve the purpose of prevention and treatment of asthma.

关 键 词：支气管哮喘 黄芪 一氧化氮 白细胞介素5 炎症细胞 

分 类 号：R28[医药卫生—中药学]