GAP-43治疗大鼠完全性脊髓损伤后Nogo-A在神经元的表达  

The Changes of Nogo-A Expression in the Neurons of Acute Complete SCI Rats after GAP-43 Therapy

作　　者：段艳萍[1] 黄素群 朱琳超 王红梅[3] 冯林森[3] 王金德[4] 

机构地区：[1]昆明医学院组织学与胚胎学教研室,云南昆明650500 [2]海源学院,云南昆明650106 [3]昆明医学院 [4]昆明医学院人体解剖学教研室,云南昆明650500

出　　处：《昆明医学院学报》2011年第12期21-25,共5页Journal of Kunming Medical College

基　　金：云南省教育厅自然科学基金资助项目(JYT0905F);云南省教育厅课题(09C0017);云南省教育科学规划课题(GY09006)

摘　　要：目的制备完全性脊髓损伤成年SD大鼠模型,研究生长相关蛋白(GAP-43)治疗大鼠脊髓损伤后神经元上Nogo-A的变化,探讨GAP-43在再生修复中的作用,为临床治疗提供实验信息.方法雌性8周龄SD大鼠75只,制成脊髓损伤模型,随机分为3组:GAP-43抗体组,GAP-43抗原组,对照组,每组25只.使用直接注射法将GAP-43抗原和GAP-43多克隆抗体分别注入抗原组和抗体组的大鼠脊髓断端,观察各组大鼠肢体功能的恢复情况,用BBB评分法进行不同时段的行为学评分;用HE染色和免疫组化染色及图像分析方法观察Nogo-A阳性神经元数目的变化,并对其进行相关性分析.结果对照组在不同的时间段行为学评分最低,抗原组评分最高,抗原组Nogo-A阳性神经元数量最少,且数量与后肢功能恢复呈反相关.结论说明GAP-43能够与Nogo-A相拮抗,促进损伤脊髓的恢复,对脊髓损伤的研究与治疗具有重要的意义.Objective To provide evidence for the therapy effect of neuronal factor GAP-43 after spinal cord injury by observating the expression changes of Nogo-A in complete spinal cord injury rats.Methods Seventy-five 8-week-aged female rats were divided into three groups randomly:the GAP-43 antibody therapy group,the GAP-43 antigen therapy group and control group,with 25 rats in each group.After GAP-43 antibody or antigen being injected into the sectioned sites of the damaged spinal cord,the behavioral scores of the rats were evaluated at different time,while H-E staining and immunohistochemical staining were used to examine the expression of Nogo-A in the damaged spinal areas.Results The results showed that the BBB score was the lowest in the control group and the highest in the antigen group,in which remarkable recovery of pathological changes of spinal cord was observed.The positive neurons'amount of Nogo-A was fewer than other groups and had negative correlation with the physical reconstruction.Conclusion GAP-43 has an antagonism with Nogo-A and has the potent neuro protective effects in the therapy for SCI.

关 键 词：脊髓损伤 GAP-43 NOGO-A 

分 类 号：R65[医药卫生—外科学]