应用化学发光免疫分析法检测HCV抗体与其他方法的比较  

To Apply Chemiluminescence Immunoassay for HCV Antibody Detection and Compared it with Other Methods

作　　者：姚仁南[1] 陈复兴[1] 陈玲[1] 蔡信海 王永方 陈娜云[1] 赵飞[1] 

机构地区：[1]解放军第97医院 [2]73083部队卫生队

出　　处：《临床输血与检验》2011年第3期200-202,共3页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：南京军区医学科技创新重点课题(No.08Z009)资助

摘　　要：目的比较化学发光免疫分析(CLIA)法、酶联免疫吸附试验(ELISA)法、胶体金法检测丙型肝炎病毒(HCV)抗体的效果。方法对80份疑似HCV感染的血清标本,分别采用CLIA法、ELISA法试剂1、试剂2和胶体金法检测。结果 CLIA法、ELISA法(试剂1、试剂2)和胶体金法检测阳性率分别为91.25%、80.63%(试剂182.50%、试剂278.75%)和56.25%。结论 CLIA法检出灵敏度显著高于ELISA法和胶体金法。Objective To evaluate the results of chemiluminescence immunoassay(CLIA) method,anti-HCV ELISA method and colloidal gold method on detection of hepatitis C virus(HCV).Method We collected 80 of suspected infected HCV serum samples.The sera were detected by CLIA,ELISA reagents ①and ② and colloidal gold reagent.Results The positive rates were respectively 91.25% by CLIA,80.63%(reagents ①82.50%,reagents ②78.75%)by ELISA and 56.25% by colloidal gold method.Conclusion The detection rate of CLIA method was significantly higher than ELISA and colloidal gold assay.

关 键 词：丙型肝炎病毒 化学发光免疫分析法 酶联免疫吸附试验 胶体金法 

分 类 号：R51[医药卫生—内科学]