Developmental changes in hemolymph ecdysteroid level and prothoracicotropic hormone activity during the fifth larval instar of the Eri silkworm, Samia cynthia ricini  

作　　者：RONG-JING JIANG HONG TENG JAN KOOLMAN SHENG LI MEI-XUN CAO 

机构地区：[1]Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,Shanghai, China [2]Institute of Physiological Chemistry, Philipps University of Marburg, Marburg, Germany

出　　处：《Insect Science》2005年第4期241-247,共7页昆虫科学（英文版）

摘　　要：Developmental changes in hemolymph ecdysteroid level, ecdysteroid synthesis by prothoracic glands （PGs） in vitro, prothoracicotropic hormone （PTTH） activity in brain extracts, and PTTH activity in the hemolymph were measured during the fifth larval instar of the Eri silkworm, Samia cynthia ricini. The changing patterns of hemolymph ecdysteroid level and ecdysteroid synthesis by laGs in vitro are similar to each other, with maximums on day 9. However, on this day, hemolymph ecdysteroid level was substantially higher than ecdysteroid synthesis by PGs in vitro suggesting a high PTTH activity in the hemolymph on day 9. Moreover, the changing pattern of PTTH activity in brain extracts is also similar to that of PTTH activity in the hemolymph, both peaking on day 9. However, on this day, activity in brain extracts was much smaller than PTTH activity in the hemolymph implying that most PTTH synthesized by the brain is secreted to the hemolymph and the brain stores a very little amount of PTTH. This study provides unique insights onto the hormonal regulation of ecdysteroid synthesis in the Eri silkworm and is useful for our future studies on signal transduction of insect neurolaelatides.在血淋巴 ecdysteroid 的发展变化铺平，由专业版的 ecdysteroid 合成胸的腺(PG ) 试管内，在大脑摘录的 prothoracicotropic 荷尔蒙(PTTH ) 活动，和在血淋巴的 PTTH 活动在 Eri 的第五幼虫的中间形态期间被测量， Samia 月之女神篦麻毒素 i。由 PG 试管内的血淋巴 ecdysteroid 水平和 ecdysteroidsynthesis 的变化模式类似于对方，与白天 9 上的最大值。在这天，然而，血淋巴 ecdysteroid 水平比由在 vitrosuggesting 的 PG 的 ecdysteroid 合成实质地高在白天 9 上的血淋巴的一项高 PTTH 活动。而且，在大脑摘录的 PTTHactivity 的变化模式也类似于在血淋巴的 PTTH 活动的，两个都在白天 9 上达到顶点。在这天，然而，在大脑摘录的 PTTH 活动是比在暗示大多数 PTTH 用大脑综合了的血淋巴的 PTTH 活动小得多的被分泌到血淋巴，大脑存储 PTTH 的很小的数量。这研究在 Eri 提供唯一的卓见到 ecdysteroid 合成的神经质的规定上并且为我们昆虫 neuropeptides 的信号转导上的未来研究是有用的。

关 键 词：Samia cynthia ricini ECDYSTEROIDS prothoracic gland prothoracicotropic hormone RADIOIMMUNOASSAY 

分 类 号：Q969.435.1[生物学—昆虫学]