吗啡预处理对成年大鼠心肌细胞缺血-再灌注损伤的保护作用  

Effect of morphine preconditioning on ischemia-reperfusion injury in cultured adult rat cardiac myocytes

作　　者：李洪[1] 肖颖彬[2] 杨天德[1] 方国新[1] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆市400037 [2]第三军医大学新桥医院心血管外科,重庆市400037

出　　处：《临床麻醉学杂志》2005年第8期546-548,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金资助课题(No30200089)

摘　　要：目的探讨吗啡预处理对培养大鼠心肌细胞模拟缺血-再灌注损伤的保护作用及其与剂量的关系.方法采用培养成年大鼠心肌细胞模拟缺血-再灌注损伤模型,观察不同浓度的吗啡预处理对心肌细胞缺血-再灌注损伤后细胞成活率和肌酸激酶(CK)释放的影响.结果吗啡(0.1～10μmol/L)预处理培养大鼠心肌细胞后,能显著减少模拟缺血-再灌注损伤引起的心肌细胞死亡和CK的释放(P＜0.01).在0.1～1 μmol/L的浓度范围内,吗啡预处理对心肌细胞的保护效应呈剂量依赖性增加.结论在0.1～1μmol/L的浓度范围内,吗啡预处理能剂量依赖地产生心肌保护效应.这提示吗啡在整体心脏预处理心肌保护效应可在成熟的心肌细胞水平起作用.Objective To investigate the relationship between dose of morphine and protection of morphine preconditioning from simulated ischemia-reperfusion injury in cultured adult rat cardiac myocytes. Methods In the model of cultured adult rat cardiac myocytes suffered from simulated ischemia-reperfusion injury, the myocytes were pretreated with morphine in concentration from O. O1 μmol/L to 10 μmol/I.,and then simulated ischemia for 40 rain and reperfused for 30 rain. The cell viability and creatine kinase（CK） released from the cells were measured by MTT and CK test kit respectively. Results Morphine pretreatment was able to protect cardiomycytes against simulated ischemia-reperfusion injury by increasing the number of live cells and decreasing the release of CK from the cardiomyocytes. In the concentrations from 0.1 μmol/L to 1 μmol/L, the effects of morphine pretreatment on protecting cardiomyocytes against simulated ischemia-reperfusion injury were increased in a dose-dependent manner. Conclusion Morphine （0.1-1 μmol/L） can dose-dependently protect the cardiomyocytes from simulated ischemia-reperfusion injury. The results provide a direct evidence that the preconditioning-like effect of morphine in the intact heart can be exerted in adult cardiomyocytes.

关 键 词：吗啡 预处理 成年 大鼠 心肌细胞 缺血-再灌注损伤 肌酸激酶 保护作用 

分 类 号：R541[医药卫生—心血管疾病]