缺氧时15-脂氧化酶在体外牛肺动脉内皮细胞的表达  

Expression of 15-lipoxygenase in endothelial cell of hypoxic bovine pulmonary artery in vitro

作　　者：金明顺[1] 刘慧雯[1] 叶宏[2] 张翠香[1] 单智焱[1] 刘东华[1] 朱大岭[2] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学药学院,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2005年第4期311-313,共3页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(30370578)

摘　　要：目的探讨急性缺氧时,15-脂氧化酶(15-LO)在体外肺动脉内皮细胞的表达部位及程度。方法通过细胞培养,然后牛肺动脉内皮细胞分别缺氧0h、0.5h、1h、2h、3h,在倒置显微镜下观察细胞形态,通过做免疫组化,观察牛肺动脉内皮细胞15-LO的表达情况及表达部位。结果正常情况下,牛肺动脉内皮细胞,倒置显微镜下呈扁平或多角形。通过免疫组化实验可观察到,15-LO在牛肺动脉内皮细胞胞质内有表达,而且在不同缺氧时间均有表达, 15-LO被染成褐色,缺氧0h时胞质内无褐色颗粒。结论缺氧条件下,在体外牛肺动脉内皮细胞15-LO确有表达, 而且随缺氧时间增加,表达增强。Objective To observe the expression of 15-1ipoxygenase（15-LO） in hypoxic pulmonary artery endothelial cell （PAEC） of the OX in vitro .Methods By inverted microscope to observe PAEC of OX in vitro and to make hypoxic model for 0h,0.5h, lh,2h and 3h, then immunohistochemistry technique was performed on cells of normal and hypoxic to observe expression location and degree of 15-LO of PAEC of OX in vitro. Results Hypoxic caused 15-LO expression in cytoplasm of PAEC of OX and no expression in normal PAEC. 15- LO were stained brown. In addition, the expression occured according to different times. Conclusion Under the condition of hypoxia, there really are expression of 15-LO in PAEC. The longer of hypoxia, the more of expression.

关 键 词：缺氧 15-脂氧化酶 牛 肺动脉 内皮细胞 

分 类 号：Q-332[生物学]