细胞间黏附分子-1与血管细胞间黏附分子-1在血管慢性排斥反应中的表达及霉酚酸酯的抑制作用  

Expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule in the chronic rejection and the inhibitory effects of mycophenolate mofitil

作　　者：仇宇[1] 祝青国[1] 陈照彦[1] 高治忠[1] 李彬彬[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院泌尿外科,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2005年第4期317-319,322,共4页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金资助项目(D9902)

摘　　要：目的探讨大鼠同种异体腹主动脉移植慢性排斥反应期间移植动脉细胞间黏附分子-1(ICAM-1)及血管细胞黏附分子-1(VCAM-1)的表达及霉酚酸酯(MMF)对移植动脉排斥反应的抑制作用。方法建立大鼠腹主动脉移植模型,设Wistar到Wistar大鼠的同系腹主动脉移植对照组、SD大鼠到Wistar大鼠的同种移植组、同种移植MMF治疗组和抗黏附分子单克隆抗体治疗组。采集移植动脉组织标本行免疫组织化学和组织病理学检查,对移植动脉组织ICAM-1及VCAM-1的表达进行定量检测。结果对照组移植动脉组织ICAM-1、VCAM-1表达较弱;同种移植组在排斥反应期间移植动脉组织血管内皮细胞的ICAM-1、VCAM-1表达强度和数量明显增加,且伴有大量淋巴细胞浸润;MMF治疗组移植动脉仅微弱表达ICAM-1、VCAM-1,同时少有淋巴细胞浸润,与黏附分子单克隆抗体治疗相似。结论ICAM-1、VCAM-1的表达水平与慢性排斥反应的发生和发展有关;MMF能显著抑制移植肾ICAM-1和VCAM-1 的表达和淋巴细胞的浸润,明显延长移植物的存活。Objective To investigate the expression of intercellular adhesion moleeule-1（ICAM-1） and vascular cell adhesion molecule （VCAM-1） in abdominal aorta tissues during the course of the chronic rejection and the inhibitory effects of mycophenolate mofitil （MMF） on the rejection of artery allograft. Methods The rat model of artery allograft was developed. The rats were divided into following groups：control group of artery allograft of Wistar to Wistar rats, group of artery allograft of SD to Wistar rats, MMF-treated group of artery allograft and antiadhesion molecule monoclonal antibody-treated group of artery allograft. The grafts were collected to receive immunohistochemical and histopathological examinations. Quantitative measurement of ICAM-1 and VCAM-1 expression in the grafts was done with computer analysis system. Results Faint ICAM-1 and VCAM-1 expression was observed in the control group. Controversially, in the allotransplantation groups, artery endothelial cells of the grafts strongly expressed ICAM-1 and VCAM-1 during the episode of rejection. Faint ICAM-1 and VCAM-1 expression and rare infiltration of lymphocytes was observed MMF-treated groups, anti-adhesion molecule monoclonal antibody-treated group as well. Conclusion The expression level of ICAM-1 and VCAM-1 is related to the occurrence and development of rejection. MMF could reduce the expression of intercellular adhesion molecule and the infiltration of lymphocytes in the grafts obviously and prolong grafts survival greatly.

关 键 词：大鼠 动脉移植 细胞间黏附分子-1 血管细胞黏附分子-1 慢性排斥 

分 类 号：R392.4[医药卫生—免疫学]