免疫抑制剂对小鼠胸腺及外周血、脾脏T淋巴细胞亚群的影响  

Effect of immunosuppressant on T cell subpopulations in thymus, peripheral blood and spleen of mice

作　　者：李霞[1] 李殿俊[1] 富东旭[1] 

机构地区：[1]哈尔滨医科大学免疫学教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2005年第4期325-326,330,共3页Journal of Harbin Medical University

摘　　要：目的研究环磷酰胺与氢化可的松对小鼠胸腺及外周血、脾脏T细胞亚群的影响。方法以环磷酰胺与氢化可的松分别注射小鼠,采用流式细胞仪分析小鼠胸腺、脾及外周血CD4、CD8细胞亚群。结果环磷酰胺组胸腺CD4、CD8双阳性细胞显著下降,氢化可的松组外周血及脾CD4单阳性细胞显著下降。结论环磷酰胺主要影响小鼠胸腺T细胞亚群,氢化可的松主要影响小鼠外周血及脾脏T细胞亚群。Objective To investigate the effect of cyclophosphamide and hydrocortisone on T cell subpopulations in thymus, peripheral blood and spleen of mice. Methods Cyclophosphamide and hydrocortisone were injected into the mice respectively and the number of T cell subpopulations in thymus, peripheral blood and spleen of mice were determined by flow cytometry, using the method of direct immunofluorescence. Results Cyclophosphamide reduced strongly CD4^＋ , CD8^＋ thymocyte subpopulation and hydrocortisone administration induced a significant decrease in the percentage of blood CD4^＋ cells in peripheral blood and spleen. Conclusion Cyclophosphamide has impact on thymocyte subpopulation, hydrocortisone has impact on peripheral blood and spleen T lymphocytes.

关 键 词：免疫抑制剂 环磷酰胺 氢化可的松 T细胞亚群 

分 类 号：R392.12[医药卫生—免疫学]