低脂膳食对胰岛素抵抗大鼠脂肪组织蛋白激酶B表达的影响  

EFFECT OF LOW FAT DIET ON EXPRESSTION OF PTOTEIN KINASE B IN ADIPOSE TISSUE OF RATS WITH INSULIN RESISTANCE

作　　者：毕会民[1] 李建辉[1] 汪保华[2] 

机构地区：[1]武汉大学人民医院内分泌科,武汉430060 [2]荆门市第一人民医院神经内科,荆门448000

出　　处：《营养学报》2005年第4期288-291,共4页Acta Nutrimenta Sinica

基　　金：湖北省自然科学基金资助(No.2003ABA182)

摘　　要：目的:在高脂饮食诱导胰岛素抵抗的基础上,观察低脂膳食对胰岛素抵抗大鼠脂肪组织中蛋白激酶B(proteinkinaseB,PKB)表达的影响。方法:雄性Wistar大鼠30只,随机分为正常对照组(controlgroup,C)10只,给予低脂饲料;模型组(modelgroup,M)20只,给予高脂饲料。喂养4w后,又随机分为2组:高脂喂养组(highfatdiet,HF),继续高脂饮食;低脂喂养组(lowfatdietedgroup,LF),给予低脂饮食。干预6w后,蛋白印迹法检测大鼠脂肪组织中胰岛素刺激PKB的蛋白表达含量。结果:1.高脂M组的空腹血糖(FBG)、胰岛素(FINS)、甘油三酯(TG)、胆固醇(TC)及胰岛素抵抗指数(homeostasismodelassessment-insulinresistanceindex,HOMA-IR)明显升高,胰岛素敏感指数(insulinresistanceindex,ISI)显著下降,出现了胰岛素抵抗。2.低脂膳食干预6w,LF组的FBG、TG、TC及HOMA-IR下降,ISI显著升高。3.长期高脂膳食,HF组大鼠脂肪组织中PKB的表达明显减少,较C组下降了23.5%。低脂饮食6w后,LF组大鼠PKB蛋白表达明显升高,较HF组增加了16.1%。结论:低脂膳食纠正糖脂代谢紊乱,改善胰岛素抵抗,可能与增加脂肪组织中PKB蛋白表达有关。Objective： To study the effect of low fat diet on expression of protein kinase B （PKB） in adipose tissue of rats with insulin resistance. Method： Thirty male Wistar rats were randomly divided into control group （ C, n=10）, given low fat diet; model group （M, n=20）, given high fat diet. After 4 w, group M was randomly divided into 2 subgroups accepting diet intervention of 6 w. （1） High fat group （HF） was continually given high fat diet; （2） Low fat group （LF） accepted low fat diet. The expression of PKB stimulated by insulin in adipose tissue was determined with Western blotting at the end of the experiment. Results： （1） Group M showed hyperglycemia, hyperinsulinism, hypertriglyceride, hypercholesterolemia and elevated HOMA insulin resistance index （HOMA-IR） while the insulin sensitivity index （ISI） was obviously reduced compared to group C, indicating that the insulin resistant model was induced ; （2） Low fat diet treatment decreased FBG, TG, TC, which were accompanied by lower HOMA-IR and elevated ISI; （3） Because of long term high fat diet. exoression of PKB in adioose tissue of grouo HF decreased bv 23.5% （ P 〈 0.01 ） compared with group C. There was an increase of 16.1% （P 〈 0.01 ） in PKB expression of group LF compared to group HF. Conclusion： Low fat diet can significantly elevate expression of PKB and ameliorate the state of insulin resistance.

关 键 词：饮食 胰岛素抵抗 脂肪组织 蛋白激酶B 

分 类 号：R587.1[医药卫生—内分泌]