流动注射化学发光法测定维生素B_1  

Determination of Vitamin B_1 by Flow Injection Analysis with Chemiluminescence Detection

作　　者：汪敬武[1] 谢志鹏[2] 杨伟平[3] 

机构地区：[1]南昌大学化学系,南昌330047 [2]江西理工大学化学系,赣州341000 [3]南昌大学医院,南昌330047

出　　处：《药物分析杂志》2005年第8期996-998,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测定维生素 B_1片剂中维生素 B_1的含量。方法:基于维生素 B_1对鲁米诺-铁氰化钾化学发光反应的增敏作用,采用盐酸对维生素 B_1进行保护,进样量为50μL,流速为2.0 mL·min^(-1)。结果:维生素 B_1的浓度在1.0×10^(-7)～1.0×10^(-6) mol·L^(-1)与1.0×10^(-6)～1.0×10^(-5)mpl·L^(-1)范围内分段回归呈良好的线性关系,检测限达到3.0×10^(-8)mol·L^(-1)。对6.0×10^(-6)mol·L^(-1)的维生素 B_1平行测定11次,RSD 为2.8%,与药典方法对比,结果基本一致。结论:方法准确可靠,已成功用于维生素 B_1片剂的测定。Objective ： To establish an FIA - CL method for the determination of vitamin B1 in medicinal tablets. Methods： It was based on the enhancement effect of vitamin B1 on the CL intensity of luminol- K3Fe（CN）6 system. The vitamin B1 was protected by HC1 solution. The flow rate was 2. 0 mL · min^-1. Injection was operated using a six - way injection valve fitted with a 50μL loop. Results： The calibration curve was linear in the range of 1.0 × 10^-7 - 1.0× 10^-6 mol · L^-1 and 1.0 × 10^-6 - 1.0 × 10^-5 mol·L^-1 respectively with a detection limit of 3.0 × 10^-8 mol·L^-1 and a sampling rate of 72 times per hour. The relative standard deviation was 2. 8% for the determination of 6. 0 × 10^-6 mol · L ^-1 vitamin B1 （ n = 11 ）. Conclusion ： The method is reliable, accurate and very suitable for the determination of vitamin B1.

关 键 词：流动注射 化学发光法 维生素B1 流动注射化学发光法 测定 鲁米诺-铁氰化钾 化学发光反应 增敏作用 线性关系 分段回归 

分 类 号：R977.22[医药卫生—药品] R987[医药卫生—药学]