Analysis of the Cotton E6 Promoter  

作　　者：吴蔼民 刘进元 

机构地区：[1]Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China

出　　处：《Tsinghua Science and Technology》2005年第4期409-413,共5页清华大学学报（自然科学版（英文版）

基　　金：Supported by the National High-Tech Research and Development (863) Program of China (Nos. 2001AA222053, 20O2AA212051, and 2002AA207006) and the National Natural;Science Foundation of China (No. 30270753) To whom correspondence should be addressed.

摘　　要：An E6 gene from sea island cotton （Gossypium barbadense） was expressed specifically in cotton fiber cells to transfer functions to cultivated species for better transgenic engineering. The regulatory activity of the E6 promoter region was then studied by isolating a 614-bp fragment of the 5＇-flanking region from upland cotton （Gossypium hirsutum CR1-12） to produce a green fluorescent protein （GFP） reporter construct for analysis of tissue-specific expression in transgenic tobacco seedlings. Fluorescent analyses indicate that the relatively short E6 promoter is sufficient to direct green fluorescent protein expression specifically in the leaf trichomes （hair cells） of the transgenic tobacco plants. As cotton fibers are also unicellular trichomes that differentiate from epidermal cells of developing cotton ovules, the result suggests that the relatively short E6 promoter can serve as a fiber-specific expression promoter for genetic engineering to improve cotton fiber quality.An E6 gene from sea island cotton （Gossypium barbadense） was expressed specifically in cotton fiber cells to transfer functions to cultivated species for better transgenic engineering. The regulatory activity of the E6 promoter region was then studied by isolating a 614-bp fragment of the 5＇-flanking region from upland cotton （Gossypium hirsutum CR1-12） to produce a green fluorescent protein （GFP） reporter construct for analysis of tissue-specific expression in transgenic tobacco seedlings. Fluorescent analyses indicate that the relatively short E6 promoter is sufficient to direct green fluorescent protein expression specifically in the leaf trichomes （hair cells） of the transgenic tobacco plants. As cotton fibers are also unicellular trichomes that differentiate from epidermal cells of developing cotton ovules, the result suggests that the relatively short E6 promoter can serve as a fiber-specific expression promoter for genetic engineering to improve cotton fiber quality.

关 键 词：cotton E6 promoter green fluorescent protein florescence trichome-specific expression transgenic tobacco 

分 类 号：S562[农业科学—作物学]