吉林省部分水稻品种稻瘟病菌Pot2指纹分析  

Pot2 Fingerprint of Magnaporthe grisea DNA from Some Rice Varieties in Jilin Province

作　　者：任林柱[1] 王庆钰[1] 白容霖[2] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]中国人民解放军军需大学农副业生产系,长春130062

出　　处：《吉林农业大学学报》2005年第4期369-372,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(39770483)

摘　　要：采用Pot2-rep-PCR指纹图谱法分析了吉林省部分水稻品种稻瘟病组织,发现所有病组织都能扩增出条带,扩增的片段长度为0.5～3.0kb,扩增的DNA片段数为1～6,而且各病组织在约600bp处共有1条带.经聚类分析,在80%遗传相似水平下,可将供试的15个样品分为7个谱系.不同地域同一水稻品种上的稻瘟病菌生理小种指纹不同.DNA samples of diseased rice tissue collected from Jilin province were analyzed by Pot2 - rep - PCR with two outwardly directed primers of Pot2, an element found in approximately 100 copies in Magnaporthe grisea genome. We found that the molecular weight of fragments ranged from 0.5 to 3.0 kb, the number of amplification fragments was 1 to 6, and there was one common band in all DNA fingerprints. The isolates were divided into 7 lineages according to the DNA band data at the level of 80 % similarity. The fingerprints of physiologic races collected from the same variety of different districts were different.

关 键 词：稻瘟病菌 Pot2-rep-PCR扩增 指纹图谱 吉林省 水稻 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]