串联表达传染性喉气管炎病毒gB、gD抗原表位基因的重组鸡痘病毒转移载体的构建  

Construction of Recombinant Fowl Poxvirus Transfer Vector Tandem Expressing the Antigen Epitope Genes of Glycoprotein B and Glycoprotein D of Infectious Laryngotractheitis Virus

作　　者：刘文波[1] 梁成珠 徐彪 朱来华 张素芳[1] 陈溥言[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京210095 [2]青岛出入境检验检疫局,青岛266002

出　　处：《吉林农业大学学报》2005年第4期453-456,460,共5页Journal of Jilin Agricultural University

基　　金：江苏省兽用生物制品工程中心开放项目资助(02-432)

摘　　要：根据已发表的传染性喉气管炎病毒(ILTV)SA-2株基因序列设计并合成2对引物,以烟台分离株为模板,分别扩增其gB和gD的抗原表位基因,并亚克隆到pBluescriptⅡSK(+)中,得到重组质粒pSK-gB-gD,然后再将gB-gD片段亚克隆到鸡痘病毒转移质粒载体pEFgpt12s中。酶切鉴定结果显示已成功构建了重组鸡痘病毒转移质粒载体pEF-gB-gD,可为进一步在细胞中转染并获得串联表达传染性喉气管炎病毒gB及gD主要抗原表位基因的重组鸡痘病毒奠定基础。Two pairs of primers were designed according to the nucleotide sequence of the ILTV SA-2 strain published in the GenBank. One was used to amplify the antigen epitope genes of gB and the other was used to amplify the antigen epitope genes of gD. The amplified gB and gD genes were subcloned into the pBluescript Ⅱ SK （ ＋ ） and pSK - gB - gD was constructed. Then the pSK - gB - gD was digested with XbaI and PstI and the obtained gB - gD fragments were subcloned into pEFgpt12s, a recombinant fowl poxvims transfer vector. The identification results by digesting the recombinant plasmid with restriction endonucleases showed that the transfer vector pEF - gB - gD containing the major antigen epitope genes of gB and gD of ILTV was constructed successfully and it will be used to select the recombinant fowl poxvirus.

关 键 词：传染性喉气管炎病毒 糖蛋白B 糖蛋白D 鸡痘病毒 

分 类 号：R392.11[医药卫生—免疫学]