柔嫩艾美耳球虫杂交株MIC2基因克隆及序列分析  

Cloning and Sequencing of MIC2 Gene from Hybridization Strain of Eimeria tenella

作　　者：王静敏[1] 魏峰[2] 张西臣[2] 蔡亚男[3] 刘全[2] 吴涛[2] 李建华[2] 尹继刚[2] 

机构地区：[1]长春税务学院信息管理系,长春130021 [2]吉林大学畜牧兽医学院,长春130062 [3]吉林农业大学生物技术学院,长春130118

出　　处：《吉林农业大学学报》2005年第4期457-460,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(30170696)

摘　　要：根据柔嫩艾美耳球虫子孢子微线蛋白2的cDNA序列,利用计算机设计1对引物,以柔嫩艾美耳球虫杂交株子孢子总RNA为模板,利用RT-PCR方法扩增出1个片段。把这个片段克隆到pMD18-T载体,经酶切鉴定得到1个阳性克隆,测序及序列分析结果表明该片段为MIC2基因,开发阅读框(ORF)与E.tenella豪顿株MIC2核苷酸同源性为99.51%,推导的氨基酸序列同源性为99.12%,说明MIC2基因高度保守。The MIC2 gene of E.tenella hybridization strain was amplified with the primers designed according to MIC2 gene cDNA of E.tenella and cloned into pMD18T vector. The recombinant plasmid was identified by restriction endonuclease EcoR Ⅰ/Hind Ⅲ and sequenced. The results indicated that the MIC2 gene of E.tenella hybridization strain including 1029 bp nucleotides was successfully cloned and there were only five nucleotides different from that of E.tenella Horton strain and it was very conservative in that the homogeneity of nucleotide and amino acid was respectively 99.51% and 99.12%.

关 键 词：柔嫩艾美耳球虫 微线蛋白 基因克隆 序列分析 

分 类 号：S852.723[农业科学—基础兽医学]