MMP-9及TIMP-1在子宫内膜异位症患者腹水及血清中的表达  

Expressions of MMP-9 and TIMP-1 in peritoneal fluid and sera of patients with endometrosis

作　　者：李艳[1] 郎景和[1] 王立杰[2] 许秀英[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,北京100730 [2]山东大学齐鲁医院妇产科

出　　处：《现代妇产科进展》2005年第4期293-296,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(39830350)

摘　　要：目的:从蛋白水平检测内异症患者血清和腹水中MMP9及TIMP1浓度,探讨MMP9、TIMP1及腹水微环境在内异症发病中的作用。方法:收集47例RAFSI～IV期内异症患者的腹水,30例非内异症患者的腹水;从血清库中获取27例内异症患者血清,并收集26例正常妇女的血清作为对照。用ELISA法检测血清及腹水MMP9及TIMP1水平。结果:内异症患者腹水TIMP1与非内异症对照组无明显差异,但MMP9的水平明显高于对照组;内异症患者血清中MMP9的浓度与正常对照组差异有显著性,血清TIMP1的水平与对照组无明显差异,血清中MMP9/TIMP1的比值明显高于对照组;内异症患者血清MMP9浓度明显比腹水高,而TIMP1浓度明显比腹水低,因而血清中MMP9/TIMP1的比值是腹水的700倍左右。结论:MMP9及TIMP1参与内异症的发病,MMP9/TIMP1比值的测定具有临床意义;腹水微环境和内异症发生发展相互影响;血清的蛋白水解潜能远远大于腹水,其意义和解释需要进一步研究。Objective：To detect concentrations of MMP-9,TIMP-1 in sera and peritoneal fluid of women with endometriosis,and compare them to disease-fess controls.Methods：Seventy-seven specimens of peritoneal fluid（PF）from patients（47 with endometriosis and 30 without endometriosis）were collected at the time of laparoscopic surgery.Sera specimens of 27 patients with endometriosis（EM）wer obtained from sera bank.26 normal women were selected as control group,and their sera were collected.MMP-9 and TIMP-1 concentrations of PF and sera were measured by the method of ELISA.Results：Concentrations of MMP-9 and ratio of MMP-9/TIMP-1 of PF from patients with endometriosis were significantly higher than those with patients without this disease.MMP-9 concentrations of sera from patients with EM were significantly higher than those from the control group.MMP-9 concentration of sera was much higher than that of PF,while TIMP-1 level of sera was much lower than that of PF,therefore,the ratio of MMP-9/TIMP-1 in sera was about 700 times of that in PF.Conclusion：MMP-9 and TIMP-1 participate in the onset of EM,may play a role in the developmet of EM.The results also indicate the relevance between PF microenvironment and EM.The proteolytic capacity in sera is much higher than in PF,but the significance and explanation need to be further investigated.

关 键 词：子宫内膜异位症 基质金属蛋白酶 金属蛋白酶1组织抑制剂 腹水 血清 

分 类 号：R711.32[医药卫生—妇产科学]