猪卵巢重量与卵母细胞质量的相关性研究  

The research on the relation between weight of ovary and quantity of the porcine oocytes

作　　者：黄德宝[1] 胡建宏[1] 李青旺[1] 冯涛[1] 丁海荣[1] 杨智青[1] 董文素[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]燕山大学附属中学,河北秦皇岛066004

出　　处：《西北农林科技大学学报（自然科学版）》2005年第8期12-15,20,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河北省秦皇岛市科技局项目"利用转基因羊生产基因工程药物"部分内容(DT83)

摘　　要：将132个猪卵巢按重量分为A、B和C 3个级别,用抽吸法结合切剖法采集卵母细胞,研究卵巢重量与3个级别卵母细胞质量的相关性.结果表明:当卵巢重量>4.000 g时,A级卵母细胞的数量与卵巢重量呈负相关(rA=-0.277 0);当卵巢重量在2.000～4.000 g时,A级卵母细胞的数量与卵巢重量呈正相关(rA=0.382 7),且差异极显著(P<0.01),无论单个卵巢获得的卵母细胞总数,还是A级卵母细胞的比例,均明显高于其他重量的卵巢;当卵巢重量≤2.000 g时,A级卵母细胞的数量与卵巢重量有一定的正相关性.研究结果表明,在采集猪卵巢卵母细胞时,建议采集重量在2.000～4.000 g的卵巢,以获得较多数量的A级卵母细胞.Aspiration and dissection were used to dispose 132 collected pig ovaries in this paper. The ovaries were divided into three classes（m≤2. 000 g,2. 000 g〈m〈4. 000 g,m〉4. 000 g） according to the weight （m） and the quantity of oocytes. Then the data were analysed. The result demonstrated, at rA〉 4. 000 g,the quantity of A-class oocytes and ovary weight had a negative correlation（rA =-0. 277 0）; 2. 000-4. 000 g, the quantity of A-class oocytes and ovary weight had a positive correlation obviously, rA=0. 382 7（P〈0.01）. In the weight range ,the average of the oocytes from each ovary or the percent of the Class-A were more than other weight ranges;at m≤2. 000 g,the quantity of A-class oocytes and ovary weight had a positive correlation. Ovaries with the weight of 2. 000 g〈m〈4. 000 g should be collected,so that more Class-A oocytes can be obtained.

关 键 词：猪 卵巢重量 卵母细胞质量 相关性 

分 类 号：S828.34[农业科学—畜牧学]