减蛋综合症病毒六邻体蛋白基因的克隆与序列分析  

Cloning and sequence analysis of hexon-encoding gene of egg drop syndrome virus

作　　者：李银聚[1] 吴庭才[1] 程相朝[1] 吴志明 张春杰[1] 

机构地区：[1]河南科技大学动物科技学院 [2]河南省兽医防治站,河南郑州450002

出　　处：《西北农林科技大学学报（自然科学版）》2005年第8期31-34,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河南省自然科学基金资助项目(0214040027)

摘　　要：设计了1对特异性引物,应用降落PCR法对减蛋综合症病毒(EDSV)六邻体蛋白基因进行了扩增。将扩增产物克隆至pMD18-T载体中,经酶切和PCR鉴定后,初步证明了目的片段的正确性。进一步经核苷酸序列测定表明,所扩增的基因片段长度为2.74kb,共编码910个氨基酸,与由EDSV弱毒株(AA-2)全基因组的Hind酶切片段测序所得到的六邻体结构基因推测的氨基酸序列相比,有11处氨基酸发生了变异。Hexon-ecoding protein gene of egg drop syndrome virus （EDSV） was amplified by touchdown PCR（TD-PCR） and was cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The sequence analysis showed that the nucleotide sequence of hexon protein gene was 2.74 kb and encoding a protein of 910 amino acids, 11 amino acids were different from the hexon protein sequenced from the Hind Ⅲ -D fragment of egg drop syndrome virus AA-2 strain.

关 键 词：减蛋综合症病毒 六邻体蛋白基因 克隆 序列分析 

分 类 号：S851.347.201[农业科学—预防兽医学]