大豆疫霉的分子检测  

Molecular detection of Phytophthora sojae

作　　者：陈长卿[1] 康振生[1] 王晓杰[1] 黄丽丽[1] 左豫虎[2] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100 [2]黑龙江八一农垦大学植物科技学院,黑龙江大庆163319

出　　处：《西北农林科技大学学报（自然科学版）》2005年第8期73-77,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家重点基础研究发展规划(2002CB111406);国家杰出青年基金项目(30125031);黑龙江省自然科学基金项目(TC2005-08)

摘　　要：利用核糖体内转录间隔区(internaltranscribedspacer,ITS)序列通用引物ITS1/ITS4分别获得了大豆疫霉(Phytophthorasojae)和辣椒疫霉(P.capsici)的ITS序列,通过获得序列设计了大豆疫霉的特异性引物PS1和PS2,并建立了分子检测方法。该方法对大豆疫霉(P.sojae)菌丝体和土壤中卵孢子检测的灵敏度分别为10-5ng/μL和每克土壤0.05个卵孢子。Genetic PCR （polymerase chain reaction） products from the internal transcribed spacer （ITS） regions of the ribosomal DNA of P. sojae and P. capsici were cloned and sequenced. A pair of primers PS1/SP2 specific for P. sojae were designed according to multiple sequences analysis. PCR based on PS1/ SP2 can amplify a 96 bp product from genomic DNA of P. sojae strains ,but not other pathogens. PCR with the primers PS1/SP2 detected pathogen at a level of 10 ^-5 ng/μL or 0.05 oospore theoretically. P. sojae in soils and tissues of diseased sobean can also be targeted by the molecular probe. The PCR protocol provides a rapid and reliable tool to detect P. sojae and identify diseases.

关 键 词：大豆疫霉 ITS分析 PCR 分子检测 

分 类 号：S41-30[农业科学—植物保护]