23个玉米灰斑病菌菌株间亲缘关系的菌丝融合研究  

Anastomosis of 23 gray leaf spot pathogens

作　　者：王桂清[1] 陈捷[2] 

机构地区：[1]聊城大学农学院,山东聊城252059 [2]上海交通大学农业与生物学院,上海201101

出　　处：《西北农林科技大学学报（自然科学版）》2005年第B08期53-56,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十五"攻关课题(2001BA509B05)

摘　　要：为了有效地防治玉米灰斑病,利用菌丝融合技术对玉米灰斑病菌不同菌株间的亲缘关系进行了分析。结果表明,玉米灰斑病菌的菌丝融合现象普遍存在,菌丝融合测定能在一定程度上反映菌株间的亲缘关系及遗传多态性。根据菌丝融合的程度,可将不同菌株划分成A^F6个融合群,说明菌株间既有一定的亲和性又有明显的遗传异质性。但要准确分析菌株间的相互关系,则需进一步观察细胞核是否发生了融合,以便从遗传学水平上界定菌株间的关系。The anastomosis was studied on blood relationship of different gray leaf spot （Cereospora zeae-maydis）pathogens. The object was providing scientific basis for efficacious control gray leaf spot disease of maize. The result showed that the anastomosis of gray leaf spot pathogens is universal. The blood relationship and genetic diversity of different pathogens could be studied by anastomosis. According to the extent of anastomosis, 23 isolates could be classified into 6 anostomosis groups （A-F）,whieh indicated that affinity and genetic heteroplasmy occur among these isolates. However,to accurately analyze and determine the genetic relationship between these isolates at genetic level,further work should be done to examine whether the anastomosis of cell nucleus has occurred.

关 键 词：玉米灰斑病菌 亲缘关系 菌丝融合 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]