甜瓜枯萎病拮抗内生细菌筛选  

Selection of endophytic bacteria for suppression of melon wilt caused by Fusarium oxysporium

作　　者：高增贵[1] 李天来[2] 赵王月 陈捷[3] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161 [2]沈阳农业大学园艺学院,辽宁沈阳110161 [3]上海交通大学农业与生物学院,上海201101

出　　处：《西北农林科技大学学报（自然科学版）》2005年第B08期85-87,共3页Journal of Northwest A&F University(Natural Science Edition)

基　　金：辽宁省自然科学基金(20032087);沈阳农业大学园艺学院博士后研究项目

摘　　要：从2～5叶期甜瓜根苇部共分离纯化了内生细菌81株，平皿对峙试验结果表明，有6株内，仁细菌对甜瓜枯萎病菌的生长有明显的拮抗作用。其中Z46，Z53．Z54和Z56菌株的拮抗性较强。上述4株拮抗菌的5倍发酵滤液对镰刀菌的抑制率分别为62．8％，66．8％，63．0％．64．7％。温室内防效试验结果表明，Z56对甜瓜枯萎病的防效最高，200倍和400倍发酵液的防效分别为80．0％和60．0％，同时对奇主具有明显的促生作用。说明Z56菌株具有潜在生防功能。81 endophytic bacteria strains were obtained by isolating 2-5-leaf stage plants of 2 melon cultivars. Plate confrontation test showed that 6 bacteria strains inhibited the growth of Fusarium oxysporium. Among these ,antagonistic effect of Z46,Z53,Z54 and Z56 showed the best. Fermentation inhibition effect of the 4 antagonistic bacteria strains against the growth of pathogen were 62.8%,66.8%,63.0% and 64.7% respectively, Z56 strain could effectively control melon wilt ,the control efficacy of fermented solution diluted by 200 and 400 times were 80.0% and 60.0% in greenhouse test.

关 键 词：甜瓜枯萎病 内生细菌 拮抗性 防效测定 

分 类 号：S436.421.13[农业科学—农业昆虫与害虫防治]