枯萎病菌的PCR-RFLP分子检测和早期诊断  

Molecular detection and early diagnosis of Fusarium with PCR-RFLP program

作　　者：张宝俊[1] 李志岗[1] 郭明霞[1] 徐玉梅[1] 王建明[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《西北农林科技大学学报（自然科学版）》2005年第B08期88-90,共3页Journal of Northwest A&F University(Natural Science Edition)

基　　金：山西省留学基金(2004048)

摘　　要：采用自行设计的、能对镰刀菌18srDNA进行特异性扩增的1对引物,利用PCR-RFLP对尖孢镰刀菌以不同方式侵染的植株进行跟踪检测。结果表明,应用PCR-RFLP检测程序可检测出处于侵染初期和潜育期的枯萎病菌,说明PCR-RFLP能够快速、灵敏、准确地对枯萎病进行早期诊断。A pair of primer designed personally was used to amplify 18s rDNA of Fusarium and plants inoculated by Fusariurn oxysporum in different ways were followed by PCR-RFI.P, It showed that PCRRFLP programs can detect Fusarium in initial penetration and incubation periods ,which showed that PCRRFLP can diagnose early symptoms of Fusarium wilt disease rapidly,sensitively and exactly.

关 键 词：PCR—RFLP 尖孢镰刀菌黄瓜专化型 尖孢镰刀菌萎蔫专化型 

分 类 号：S436.421.13[农业科学—农业昆虫与害虫防治]