淡紫拟青霉种内异核现象的初步研究  

Preliminary study on the heterokaryosis of different isolates of Paecilomyces lilacinus

作　　者：王艳娜[1] 汪来发[1] 朴春根[1] 张哲峰 

机构地区：[1]中国林业科学研究院,北京100091 [2]河北省政法职业学院,河北石家庄050061

出　　处：《西北农林科技大学学报（自然科学版）》2005年第B08期157-160,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家林业局"948"引进项目(2004-4-37)

摘　　要：研究了淡紫拟青霉的不同田间分离菌株单孢子后代的异核现象。结果表明,来源于福建、江苏、安徽的4个菌株(19,20,459,641)的单孢子培养后代,在PPDA+1%活性炭的培养基上进行配接试验,仅在19和459 2个菌株间能形成异核体,其频率为12.25%;在配接试验中,2个亲和菌落交界处长出致密的菌丝簇组成的实线,可推测为异核体。从来自菌丝簇线的每个单菌丝尖端培养物中分离出30个以上的单孢子,将其分别移接到PDA平板上,将来源于单孢子的菌落与2个亲本菌落进行比较,有3个菌丝尖端培养物(459-8×19-2,459-7×19-10,459-6×19-1)重现了两亲本类型或出现了新的菌落类型,异核现象得到证实。Heterokaryosis was tested between two monoconidial progenies from different isolates of Paecilomyces lilacinus. Heterokaryotic hyphae appeared as a solid line of white mycelial tufts where contact occurred between colonies of compatible isolates when they were paired on a modified peptone potato dextrose agar plus medium containing 1% active carbon. A small mycelial fragment from the tufted zone was transferred to Petri dishes containing water agar and hyphal tip isolates were obtained from the resulting colonies. Thirty or more monoconidial isolates were further obtained from each hyphal tip isolate and plated on PDA in Petri dishes. The resulting cultures were compared with the two parental isolates that produced the tufted reaction. Heterokaryosis was demonstrated when different monoconidial isolates from hyphal tip cultures 459-8 × 19-2,459-7 × 19-10,459-6 × 19-1 were representative of both parental types and/or when new colony morphologies were recovered. One of 6 different combinations involving 4 different field isolates produced heterokaryons. Heterokaryons were formed at a frequency of 12.25% between the monoconidial progeny of isolate 459 and isolate 19.

关 键 词：淡紫拟青霉 异核现象 南方根结线虫 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]