冠心病患者血清抗G-蛋白偶联受体自身抗体的分布特性  

The distribution of autoantibodies against G protein-coupled receptors from the sera of patients with coronary atherosclerotic heart disease

作　　者：贺忠梅[1] 左琳[1] 李美霞[1] 刘慧荣[1] 汤嘉宁[2] 魏书庆[2] 

机构地区：[1]山西医科大学生理教研室,030001 [2]太原钢铁(集团)有限责任公司总医院心外科

出　　处：《中国药物与临床》2005年第8期579-581,共3页Chinese Remedies & Clinics

基　　金：山西省自然科学基金资助项目(20021103);山西省资助回国留学人员科研项目基金资助项目(200526)

摘　　要：目的探讨冠心病患者血清中抗G-蛋白偶联受体自身抗体的分布特征。方法选择冠心病患者131例,正常健康体检者100名,采用链酶亲和素-酶联免疫吸附试验(SA-ELISA)测定其血清中抗G-蛋白偶联型β1AR、Μ2R、α1AR自身抗体。结果①冠心病患者血清抗G-蛋白偶联型β1AR、Μ2R、α1AR自身抗体的阳性率分别为20.6%(27/131)、16.8%(22/131)、39.7%(52/131),明显高于正常对照组的7.0%(7/100)、8.0%(8/100)、15.0%(15/100)(P<0.05);②冠心病组抗β1AR、Μ2R、α1AR自身抗体阳性患者的平均抗体滴度分别为1:(87.3±3.1)、1:(92.5±2.4)、1:(101.2±2.6),明显高于正常对照组的1:(14.6±2.7)、1:(21.4±2.2)、1:(26.6±3.8)(P<0.01)。结论冠心病患者血清中不仅存在抗G-蛋白偶联型β1AR、Μ2R和α1AR自身抗体,而且抗体阳性率和滴度也明显高于正常对照组,提示这些自身抗体的出现及其介导的自身免疫反应可能参与冠心病的病理生理过程。Objective To investigate the distribution properties of autoantibodies against G protein-coupled β1-adrenergic receptor （β1AR） and α1-adrenergic receptor （α1AR） and M2-musearinic receptor（M2R） in patients with coronary atheroselerotic heart disease （CAHD）. Methods One hundred and thirty-one CAHD patients and 100 healthy volunteers（HC） were chosen. The autoantibodies against G protein-coupled β1AR and α1AR and M2R from the sera of the selected cases were screened by using SA-ELISA. Results ①The positive rate of anti β1AR and M2R and α1AR autoantibodies of CAHD group were 20.6%, 16.8%, 39.7%, respectively , which were significantly higher than those of HC, respectively （P〈0.05）. ② The geometric mean titers of autoantibodies against β1AR and M2R and α1AR in patients of CAHD were 1 ：（87.3 ±3.1 ）, 1 ：（92.5 ±2.4）, 1 ：（101.2±2.6）, respectively,which were shown substantially higher than those in HC （P〈0.01）. Conclusion The present study indicates that the autoantibodies against G proteincoupled β1AR and α1AR and M2R exist in sera of patients with CAHD.The positive rate and mean geometric titers of these autoantibodies are higher than those of HC. These results suggested that the presence of these autaoantibodies and the autoimmune reaction induced by these autoantiboclies may be involoved in the pathophysiological mechanisms leading to CAHD.

关 键 词：冠心病 血清 抗G-蛋白偶联受体 自身抗体 病理生理 免疫反应 

分 类 号：R541.4[医药卫生—心血管疾病]