银杉外生菌根形态观察法探讨  

Morphological Observation Methods of Ectomycorrhizas in Cathaya argyrophylla

作　　者：邢树堂[1] 李玉花[1] 瓦里奥·禄敏[1] 

机构地区：[1]东北林业大学,花卉生物工程研究所,黑龙江,哈尔滨,150040

出　　处：《北华大学学报（自然科学版）》2005年第4期348-351,共4页Journal of Beihua University（Natural Science）

摘　　要：比较分析了4种可以应用于光学和电子显微观察的方法:透明压片法、石蜡切片法、半薄切片法以及超薄切片法.结果表明,上述4种方法均可用于鉴定银杉外生菌根,但对于外生菌根的早期发育阶段,透明压片法比其他方法简单方便;对于外生菌根完整结构的观察宜采用半薄切片法.Four available methods for light or electron-microscopic observation were analyzed： clearing and staining method, paraffin section method, semi-section method and ultra-thin section method, The results indicated that all these methods were able to identify ectomycorrhizal roots of C, argyrophylla. Especially, an early developmental stage of ectomycorrhizal roots identified by the clearing and staining method is easier than other methods. Alto intact structure of ectomycorrhizal roots were observed only by semi-section method.

关 键 词：银杉 外生菌根 哈蒂氏网 形态观察 

分 类 号：S791.9[农业科学—林木遗传育种]