小麦蓝矮病植原体的分子生物学检测研究  

Molecular Detection of Phytoplasma associated with Wheat Blue Dwarf

作　　者：张荣[1] 孙广宇[1] 张雅梅[1] 张秦风[1] 赵二龙[2] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100 [2]西北农林科技大学资环学院,陕西杨凌712100

出　　处：《中国农学通报》2005年第8期318-320,共3页Chinese Agricultural Science Bulletin

基　　金：陕西省自然科学基金项目(99SM03)

摘　　要：小麦蓝矮病是中国北方小麦的重要病害。利用植原体特异引物对小麦蓝矮病带病植株总DNA进行巢式-PCR(Nested-PCR)扩增,获得1.2kb的特异性片段,从分子水平上证明小麦蓝矮病是一种植原体病害。对具有红矮和蓝矮症状的病株扩增均可扩增出目标片段,说明红矮和蓝矮症状都是由植原体引起。试验为在小麦蓝矮病分子诊断、田间介体带毒检测、寄主范围研究等提供一种快速、灵敏可靠的方法。Wheat Blue Dwarf is one of the main disease on winter wheat in Northwestern China. A 1.2kb fragment was amplified by phytoplasmas universal primers in total DNA sample extracted from diseased wheat with dark green leaves and red dwarf symptom by Nested-PCR. The result indicated molecularly that the pathogen of Wheat Blue Dwarf disease was phytoplasma. The different symptoms （dark green dwarf and red dwarf） were all caused by phytoplasmas. Nested-PCR could be as a rapid, exact technique for detecting the pathogens from wheat leaves, insect vectors and weed host.

关 键 词：小麦 蓝矮病 植原体 分子生物学 田间介体带毒检测 寄主范围 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]