白术对肝硬化大鼠胃动力和氮能神经分布的影响及意义  

Effects of Atractylodes macrocephalae on gastric emptying and nitrergic nerves in rats with hepatic cirrhosis

作　　者：朱金照[1] 许其增[1] 张志坚[1] 乐桥良[1] 

机构地区：[1]中国人民解放军南京军区福州总医院消化内科,福建省福州市350025

出　　处：《世界华人消化杂志》2005年第15期1846-1848,共3页World Chinese Journal of Digestology

摘　　要：目的:观察中药白术对肝硬化大鼠胃动力及胃肌间神经丛氮能神经的影响.方法:Wister大鼠24只随机分为肝硬化模型组,白术治疗组和对照组,采用葡聚糖蓝-2000为胃肠内标记物观察大鼠胃动力变化,NADPH-d组织化学染色及肌间神经丛全层铺片技术,观察肝硬化大鼠胃底、胃体及胃窦肌间神经丛氮能神经的变化,并对其分布进行定量分析.结果:与对照组比较,肝硬化组大鼠胃动力明显减弱(160.23±45.13vs100±17.78,P<0.01),胃体、胃窦肌间神经丛氮能阳性神经排列紊乱,染色增强,密度增加,明显高于对照组(7.35±0.95vs5.52±0.92,P<0.01或6.26±0.79vs5.28±0.78,P<0.05),胃底肌间神经丛氮能阳性神经元数量及分布无明显变化;中药治疗组肝硬化大鼠胃动力明显改善(100±17.78vs96.12±16.25),胃窦肌间神经丛氮能阳性神经明显恢复(5.39±0.84vs5.28±0.78),与对照组比较均无明显变化(P>0.05).结论:中药白术对肝硬化大鼠胃动力减退有治疗作用,其机制与改善胃窦肌间神经丛氮能神经分布有关.AIM： To observe the effects of atractylodes macrocephalae （AM） on gastric motility and nitrergic nerval system in rats with hepatic cirrhosis. METHODS： Twenty-four Wister rats were randomly divided into control group, cirrhosis model group and AM treatment group. Dextran blue-2000 was used as an internal marker to assay the gastric emptying of the rats. The nitrergic nerves in the myenteric plexuses of gastric fundus, body and antrum were observed with NADPH-d histochemical staining. RESULTS： Compared with those in control group, the gastric emptyings of rats were markedly delayed in model group （160.23±45.13 vs 100± 17.78, P〈0.01）, and the distributions and densities of myenteric nitrergic nerves in the gastric body and antrum were significantly increased （7.35 ± 0.95 vs 5.52 ± 0.92, P〈0.01; 6.26 ± 0.79 vs 5.28±0.78, P〈0.05）. No significant changes were found in the fundus. After treatment with AM, the gastric motility of rats was markedly improved and the nitrergic nerves in antrum were significantly recovered. No significant differences were observed between the rats of AM and control group （96.12 ± 16.25 vs 100 ± 17.78, 5.39 ± 0.84 vs 5.28 ± 0.78, both P〉0.05）. CONCLUSION： Atractylodes macrocephalae can improve the gastric motility and the mechanism may be related to its effect on the distributions of nitrigic nerves in the gastric body and antrum of rats with hepatic cirrhosis.

关 键 词：白术 胃动力 氮能神经 肝硬化 大鼠 WISTER大鼠 肝硬化模型 神经分布 胃窦肌间神经丛 胃肌间神经丛 

分 类 号：R285.5[医药卫生—中药学] R575.2[医药卫生—中医学]