HPLC法测定阿司匹林经皮给药贴剂中阿司匹林的含量  

Determination of Aspirin in transdermal delivery patch by HPLC

作　　者：杜洪光[1] 侯志芬[1] 柯光明[1] 张爽[2] 王树明[2] 

机构地区：[1]北京化工大学理学院,北京100029 [2]北京康倍得医药技术开发有限公司,北京100089

出　　处：《北京化工大学学报（自然科学版）》2005年第3期110-112,共3页Journal of Beijing University of Chemical Technology(Natural Science Edition)

摘　　要：建立阿司匹林贴剂含量的测定方法。乙腈为阿司匹林贴剂的萃取溶剂,用Waters公司SymmetryShieldTMRP18柱(3.9mm×150mm,5μm),流动相乙腈0.01mol·L-1的磷酸氢二钠(磷酸调pH值至2.50);体积比为25∶75;检测波长234nm;流速1.0mL·min-1。阿司匹林在3～96μg·mL-1范围内呈现良好的线性关系,r=0.9993。阿司匹林回收率为100.3%,RSD为0.42%。本方法简单、灵敏、准确,可作为该制剂内在质量的控制方法。A method for measurement of aspirin in the patch was developed with the high performance liquid chromatography （HPLC）, Acetonitrile was used as extracted solution. Symmetry Shield^TM RP18 column was adopted and the mobile phase was composed of acetonitrile-0.01 mol·L^-1 di-sodium hydrogen phosphate （adjusting pH to 2.50 with phosphoric acid）. A linearity with a good correlation coefficient was yielded from 3 to 96μg · mL^-1 for aspirin （r = 0.999 3）. The average recovery was 100.3% and RSD was 0.42%. This method is simple, sensitive and accurate, and can be used for the quality control of the patch.

关 键 词：阿司匹林 高效液相色谱法(HPLC) 经皮给药贴剂 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]