中药天年饮对衰老大鼠脑垂体细胞PCNA表达的影响  

EFFECTS OF TRADITIONAL CHINESE MEDICINE TNY ON PCNA EXPRESSION PITUITARY CELL OF AGING RATS

作　　者：庞晓静[1] 马建伟[2] 杨国军[1] 

机构地区：[1]承德医学院,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2005年第3期198-200,共3页Journal of Chengde Medical University

摘　　要：目的:观察中药天年饮(Tiannianyin,TNY)对亚急性衰老大鼠血清睾酮含量及脑垂体前叶细胞PCNA表达的影响。方法:采用D-半乳糖连续腹腔注射制作亚急性衰老大鼠模型,采用ELISA方法检测TNY灌胃前后大鼠血清睾酮含量,并应用免疫组织化学方法检测TNY灌胃前后大鼠脑垂体前叶细胞的增殖情况。结果:衰老大鼠血清睾酮含量明显下降,与正常大鼠比较差异显著(P<0.05);经TNY灌胃后其含量升高接近正常水平,与衰老大鼠相比有明显差异(P<0.05)。衰老大鼠脑垂体前叶PC-NA阳性细胞数量减少,与正常大鼠比较差异显著(P<0.01);经TNY灌胃后升高并接近正常水平,与衰老大鼠相比有明显差异(P<0.01)。结论:TNY可提高亚急性衰老大鼠血清睾酮含量及脑垂体前叶细胞PCNA表达水平,具有一定延缓衰老的作用。Objective：To study the effects of Traditional Chinese Medicine Tiannianyin （TNY） on testestrone level in serum and the PCNA expression in pituitary cell of aging rats. Methods： The model of sub-acutely aging rats were made by injecting D-gal into the abdominal cavity continually. Then the testestrone level in serum and PCNA expression in anterior pituitary cell were detected before and after taking TNY. Results： The testestrone level in serum of aging rats decreased significantly compared with normal rats（P〈0.05）, and the testestrone level increased after taking TNY （compared with aging rats： P〈0.05）. PCNA expression in anterior pituitary cell of aging rats decreased significantly compared with normal rats（P〈0.01）and after taking TNY it increased significantly （P〈0.01）. Conclusions： TNY has fairly effects on delaying senescence.

关 键 词：天年饮 衰老 睾酮 脑垂体 PCNA 

分 类 号：R322.54[医药卫生—人体解剖和组织胚胎学]