聚苯乙烯树脂固定化D-海因酶的初步研究  

Pilot study of D-hydantoinase immobilization by polystyrene resin

作　　者：贾红华[1] 倪芳[1] 万永敏[1] 韦萍[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《生物加工过程》2005年第2期41-44,57,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家 973项目 (2 0 0 3CB7160 0 0 4);国家自然科学重点基金项目 (2 0 3 3 60 10 )资助

摘　　要：D 海因酶广泛用于D 氨基酸的制备研究和生产中,目前已有许多固定化D 海因酶及含D 海因酶细胞的研究报道。尝试了不同功能基团的聚苯乙烯树脂进行D 海因酶的固定化,结果表明功能基为伯氨基和仲氨基效果较好,并选取聚苯乙烯树脂D92进行了固定化D 海因酶的研究。采用该树脂制备固定化酶的最优条件是:酶质量浓度6mg/mL、温度2 5℃、固化时间12h。所得固定化酶的最适作用温度4 5℃,最适作用pH为8 5 ,且作用温度及适宜pH较广,Km为游离酶的1 8倍,且储存稳定性、操作稳定性较好。4 5℃下半衰期为11d。D-Hydantoinases are widely used in research and production of D-amino acids. Many research results related to immobilized cells producing D-hydantoinase and D-hydantoinase were reported in recent years. Attempts on using polystyrene resins linked with various function groups as carriers were made in this paper. The results showed that the resin with primary and/or secondary amino group was of high catalytic activity and the resin 1）92 was selected. The optimal process of preparation of immobilization of D-hydantoinase was as follows ： mass concentration of cells was 6 mg/mL at 25 ℃ and was solidified for 12 h. The optimal temperature and pH of immobilized D-hydantoinase was 45 ℃ and 8.5, respectively,and the Km was 1.8 times as that of free enzyme. The performance and storage stability of immobilized D-hydantoinase were improved. The half-life of immobilized D-hydantoinase was 11 d at 45 ℃.

关 键 词：聚苯乙烯树脂 固定化 D-海因酶 

分 类 号：Q814.2[生物学—生物工程]