HPLC法测定小柴胡颗粒剂黄芩苷的含量  

Determination of Baicalin in Xiaochaihu Granules

作　　者：赵丽众[1] 杨雁[1] 

机构地区：[1]山东省立医院,济南250021

出　　处：《齐鲁药事》2005年第8期470-472,共3页qilu pharmaceutical affairs

摘　　要：目的应用高效液相色谱法测定小柴胡颗粒中黄芩苷的含量。方法本品以乙醇超声提取,提取液进行高效液相色谱分析,色谱柱YMC-PackODS-A(150×4·6mm,5μm),流动相为甲醇-水-磷酸(47∶53∶0·2);流速1·0ml·min-1;检测波长315nm。结果平均加样回收率为99·93%,RSD为1·5%(n=6)。结论该法简便、易行,灵敏度高,重现性好。Objective HPLC method was applied for the determination of Baicalin in Xiaochaihu Granules. Methods The chromatographic conditions were as follows：column was YMC-Pack ODS-A（ 150×4.6mm,5μm）, the mobile phase was methyl alcoholwater-phosphoric acid （47 ： 53 ： 0.2）, the flow rate of the mobile phase was 1.0ml. ml^-1, and the detective wavelength was 315nm. Results The average recovery was 99.93 % and RSD was 1.5 % （ n = 6）. Conclusion The method is simple and accurate with high sensitiveity and reproducibility.

关 键 词：高效液相色谱 黄芩苷 

分 类 号：TQ460.72[医药卫生—药物分析学]