高压氧对大鼠短暂脑缺血降钙素基因相关肽和β-内啡肽的影响  

Effects of Hyperbaric Oxygen on Calcitonin Gene-Related Peptide and β-Enderphin in Blood of Rats with Transient Global Brain Ischemia

作　　者：付慧芳[1] 陈瑞[1] 于培兰[1] 孙林[1] 谭延国[2] 

机构地区：[1]首都医科大学生化与分子生物学系 [2]首都医科大学附属复兴医院检验科

出　　处：《首都医科大学学报》2005年第4期435-437,共3页Journal of Capital Medical University

基　　金：北京市教委科技发展计划基金(00KJ108)资助项目

摘　　要：目的观察大鼠缺血再灌注损伤时降钙素基因相关肽(CGRP)和β-内啡肽(β-EP)的动态变化,以及高压氧(HBO)治疗对其的影响,并探讨高压氧的治疗机制。方法按随机数字表将SD大鼠分为以下3组:缺血再灌注组(I/R)、高压氧处理组(HBO)及假手术组(sham-O)。除假手术组外,其余各组以四动脉阻断法建立脑缺血再灌注动物模型,缺血20min。I/R组和HBO组分别于再灌注6h、24h、48h、96h取血,用放射免疫测定法(RIA)测定血浆CGRP和β-EP的质量浓度。结果1)6hHBO组即出现CGRP应激性升高,其CGRP明显高于6hI/R组(P<0.001)和假手术组(P<0.05);HBO组血浆CGRP恢复比相应I/R时相点快(从24h起即恢复至正常);2)96hI/R组CGRP高于假手术组(P<0.01)和6h、24h、48hI/R组(分别为P<0.01或P<0.00001);3)6hHBO组血浆β-EP一过性增高,然后逐渐下降,96hHBO组和96hI/R组血浆β-EP均明显低于假手术组(P<0.05),但96hHBO组和96hI/R组比较,差异无统计学意义(P>0.05)。结论早期HBO治疗可通过升高保护性神经肽CGRP和降低β-EP机制,减少缺血脑组织损伤;高压氧治疗的次数越多,疗效越好。Objective To observe the dynamic changes of the content of calcitonin gene-related peptide （CGRP） and β- enderphin（β-EP） after experimental cerebral ischemia in rats and the mechanism of hyperbaric oxygen（HBO） therapy. Methods Spragen Dawlay rats were randomly divided into the following groups： ischemia/reperfusion group（I/R）, I/R group treated by HBO（HBO） and sham operated group（sham-O） as control. Global brain ischemia was induced using a modified four-vessel occlusion model originally described by Pulsinelli and Buchan. Blood was collected respectively at reperfusion time of 6 h, 24 h, 48 h and 96 h after 20 min of ischemia. The content of CGRP and β-EP was measured by RIA. Results The plasma CGRP level in 6 h HBO group was significantly higher than that of 6 h I/R group（P 〈0.001） and sham-O group（ P〈 0.05）. The plasma CGRP levels of HBO group recovered quicker than I/R group of corresponding time point. The plasma CGRP level in 96 h I/R group was significantly higher than that of sham-O group（P〈0.01） and 6 h I/R, 24 h I/R, 48 h I/R group respectively（P〈 0. 000 01 or P 〈 0.01）. The plasma β-EP level in 6 h HBO group was slightly elevated and then decreased gradually. The plasma β-EP level in 96 h HBO group and 96 h I/R group significantly lower than that of sham-O group（P 〈 0.05）. There were no statistically significant difference between 96 h HBO group and 96 h I/R group （P 〉 0. 05）. Conclusion The findings demonstrate that early HBO therapy may reduce ischemia tissue damage by mechanism of increasing level of CGRP and decreasing level of β-EP. The more HBO treatment, the better therapeutic effect.

关 键 词：高压氧 脑缺血 再灌注 降钙素基因相关肽 Β-内啡肽 

分 类 号：Q593.2[生物学—生物化学]