重组人类生长激素改善蛋白质营养不良机制的研究  

Mechanisms of Recombinant Human Growth Hormone Improving Protein Malnutrition: a Study in Vitro

作　　者：文煜冰[1] 刘惠兰[1] 黄丽荣[1] 姚英[1] 

机构地区：[1]首都医科大学附属复兴医院肾脏内科

出　　处：《首都医科大学学报》2005年第4期467-470,共4页Journal of Capital Medical University

摘　　要：目的探讨重组人类生长激素(rhGH)对大鼠肝细胞所表达的部分营养指标的影响。方法应用原位二步法制备肝脏单细胞悬液后进行细胞培养,在培养液中加入rhGH,分别检测分泌性白蛋白(Alb)、转铁蛋白(Trf)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、IGF结合蛋白-1(IGFBP-1)和IGF结合蛋白-3(IGFBP-3)的表达量。结果1)rhGH组的Alb、Trf、IGF-Ⅰ均显著高于对照组,2组比较差异有统计学意义(P<0.01)。2)rhGH组的IGFBP-1明显低于对照组,而IGFBP-3则明显高于对照组,2组比较差异有统计学意义(P<0.05)。结论rhGH明显增加肝细胞的分泌性Alb、Trf、IGF-I等营养指标的合成和(或)分泌,明显抑制肝细胞对IGFBP-1的合成,显著上调IGFBP-3的表达。Objective To investigate the effects of rhGH on biosynthesis and secretion of secretory albumin, transferrin, insulin-like growth factor- Ⅰ （IGF- Ⅰ ）, insulin-like growth factor binding protein-1, -3（ IGFBP-1, IGFBP-3） of primary cultured hepatocytes and liver non-parenchymal cells of rat. Methods Rat hepatocytes and liver non-parenchymal cells are isolated by two- step procedures in situ. RhGH is added to the medium and the vehicle is added for control. Albumin and transferrin of medium are measured with sandwich ELISA. IGF- Ⅰ , IGFBP-1, and IGFBP-3 in cells lysate are analyzed by Western Blotting. Results The medium concentrations of albumin and transferrin and the IGF- Ⅰ level in cells lysate are significantly higher in rhGH group than in control group, all P〈0.01. The IGFBP-1 level in cells lysate is significantly lower and IGFBP-3 level is significantly higher in rhGH group than in control group, both P 〈 0.05. ConcIusion rhGH increases biosynthesis and/or secretion of secretory albumin, transferrin, and IGF-Ⅰ et al. And rhGH inhibits biosynthesis of IGFBP-1 in hepatocytes and enhances expression of IGFBP-3 in non-parenchymal liver cell significantly.

关 键 词：重组人类生长激素 蛋白质营养不良 肝细胞 

分 类 号：R459.1[医药卫生—治疗学]