缺氧复氧对IEC-6肠上皮细胞膜电位的影响及紫芪方的保护作用  

Protective Effect of Ziqi-liquid on Hypoxia Reoxygenation Damaged Intestinal Epithelial Cell Mitochondrial Membrane Potential in IEC-6 Intestinal Epithelial Cells~①

作　　者：史成和[1] 陆松敏[3] 聂渝琼[2] 刘建仓[3] 龙在云 李萍 

机构地区：[1]中国人民解放军第三军医大学,大坪医院野战外科研究所二室,重庆630042 [2]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071 [3]不详

出　　处：《解放军药学学报》2005年第4期241-244,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家"九七三"重点基础研究发展规划项目;No.G1999054203

摘　　要：目的观察缺氧复氧对IEC-6肠上皮细胞线粒体膜电位的影响,探讨抗休克复方中药-紫芪方对IEC-6肠上皮细胞缺氧复氧损伤的保护作用机制。方法建立IEC-6细胞缺氧复氧损伤模型;采用血清药理学方法,在细胞培养液中加入不同给药剂量获取的“紫芪方”药物血清(分别以10、20g·kg-1分为1次给药和间隔1h、2次给药,DJ-1,SJ-2);参附药物血清(间隔1h,2次给药20g·kg-1SF)及生理氯化钠血清(S)。采用紫外分光光度分析法测定乳酸脱氢酶(LDH)漏出量,以及采用激光共聚焦显微镜测定线粒体膜电位(MMP)变化。结果IEC-6细胞缺氧复氧损伤后,可致细胞LDH的漏出量明显升高,线粒体膜电位明显降低。紫芪方药物血清明显减轻上述损伤,与对照组比较,SJ-2组制备的药物血清能明显减少IEC-6细胞LDH的漏出量(P<0.01);并具有保护IEC-6肠上皮细胞线粒体膜电位的作用(P<0.01)。结论缺氧复氧可导致IEC-6肠上皮细胞LDH漏出量升高和线粒体膜电位降低,“紫芪方”药物血清可减少细胞LDH漏出量及保护线粒体膜电位,从而发挥细胞的保护作用。Aim To explore the protective effect and mechanism of Ziqi - liquid, a preparation of Chinese herbal medicine for antagonizing two- hit, containing sennn on Hypoxia Reoxygenation damaged mitochondrial membrane potential （MMP） in IEC-6 intestinal epithelial cells. Method Using sennn pharmacological method to preparc different quantities of drug＇s serum. Cultured intestinal epithelial cell-6 （IEC-6） cells were divided into eight groups （ n = 8） ： control group （C）, hypoxia reoxygenation group （H/R） and five treated groups： SF group （ two dose 20g·kg^-1 interval 1 hour）, DJ-1group（ 10g·kg^-1 ）, SJ-1 group（ two dose 10g·kg^-1 interval lhour ）, DJ-2 group（20g·kg^-1 ）, SJ-2 group. （ two dose 20g·kg^-1 interval lhour） and Saline （S） group. After 1 hour of hypoxia followed by 1 hour of reoxygenation lactate dehydrogenase was analysed by pectrocolorimeter and in SJ-2, C, S, H/R and SF group, MMP was detected by laser scanning confocal microscopy to see if there was any difference in the groups. Results There was significant difference in lactate dehydrogenase leakage between the control group and H/R group （ P 〈 0.01）.SJ-1 group was better than H/R group and S group （ P 〈 0.05）. SJ-2 group was much better than H/R （ P 〈 0.01）, Both were better than SF group and S group. After 1 hour of hypoxia followed by 1 hour of reoxygenation MMP in mitochondria was significantly decreased. These changes could be significantly lessened by serum containing Ziqi - liquid. Conclusion Sennn containing Ziqi - liquid has significant protective effect on Hypoxia Reoxygenation Induced injury of intestinal epithelial cell.

关 键 词：缺氧复氧 肠上皮细胞 紫芪方 乳酸脱氢酶(LDH) 线粒体膜电位(MMP) 

分 类 号：R965[医药卫生—药理学]