离子交换色谱法分析ATP研究  

Study on ATP Analysis by Chromatography

作　　者：王龙耀[1] 阮莉姣[1] 谢小瑜[1] 童张法[1] 雷爱祖[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《药物生物技术》2005年第4期239-241,共3页Pharmaceutical Biotechnology

基　　金：广西自治区科技攻关基金项目(桂科攻9920015)

摘　　要：文章针对ATP生产的现场检测过程,建立了一个以离子交换树脂为分离介质的色谱分析方法,并对其分析效果进行了试验研究。色谱条件为:D201树脂柱(Φ6 mm×500mm),流动相分别为pH值3.0的乙酸溶液和pH值9.0、1.5 mol/L的NaCl溶液,检测波长254 nm,流速10 ml/min,室温23±2℃。检测重复性较好, 在0.1-8 g/L范围内,ATP浓度与ATP峰面积呈较好线性关系,r=0.997 7;平均回收率为98.60%.整个色谱过程可在30min内完成。In the process of ATP production, a kind of chromatography method has been set up to analyze ATP and AMP. The separating medium is macroporous anion-exchange resin D201, and the effects of the analytic procedure have been discussed through experimental investigations. The chromatographic conditions were selected as follows： D201 resin column（φ6mm×500mm）; the mobile phase was acetic acid solution （pH3. 0） and NaC1 solution （pH9.0,1.5mol/L） ; detected at 254nm; flow rate was 10 ml/ min, and column temperature was 23±2℃. The method has good repeatability and good linearity within the range of 0.148 g/L（r=0. 9977） between CATF and ATP peak area. The average recovery was 98. 60%. The process can be completed within 30 min.

关 键 词：5’-三磷酸腺苷 色谱 分析方法 

分 类 号：Q657.7[生物学—生物物理学]