缝隙连接对双细胞动作电位的影响  

Effect of Gap Junction on the Action Potential of Paired Myocytes

作　　者：李霞[1] 张存泰[1] 刘念[1] 陈柏迪[1] 张繁之[1] 阮燕菲[1] 王琳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心血管内科,湖北武汉430030

出　　处：《中国心脏起搏与心电生理杂志》2005年第4期298-300,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：国家自然科学基金资助课题(编号30370573)

摘　　要：为探讨双细胞水平缝隙连接(GJ)对动作电位时程(APD)的影响,本实验用酶解法分离得到兔左室内膜心肌细胞,分为单细胞组及双细胞组,分别进行两个实验:①先用正常台氏液,续用含索他洛尔的正常台氏液灌流;②先用含索他洛尔,续用含索他洛尔+甘珀酸钠(GJ失耦联剂)的正常台氏液灌流。用β-escin穿孔膜片技术记录APD。结果:①正常状态下,单细胞组的APD大于双细胞组(688±89msvs666±77ms,P>0.05);索他洛尔灌流后,两组细胞APD均延长,单细胞组APD延长的程度明显大于双细胞组(305±66msvs168±51ms,P<0.01);②先予索他洛尔预灌流,再用索他洛尔加GJ失耦联剂———甘珀酸钠灌流,两组APD均延长,双细胞组APD延长的程度明显大于单细胞组(181±64msvs28±13ms,P<0.01)。结论:GJ可以对抗病理情况下APD的延长。In this study, we investigated the effect of gap junction on the action potential duration （APD） of paired myocytes. The rabbit subendocaidial ventricular myocytes were isolated by enzymes. Then the single myocytes and paired myocytes were perfused with sotalol and sotalol plus gap junction uncoupler-carbenoxolone. APD was recorded by using perforated patch clamp technique. Results ： ① In normal condition, APD of single myocytes was similar to that of paired. After perfusing with sotalol, APD of single myocytes was prolonged compaired with paired myocytes（305 ±66 ms vs 168 ±51 ms,P 〈0.01 ）. ② For those first with sotalol and then with sotalol plus carbenoxolone perfusion, APD was all lengthened in the two groups （ P 〈 0. 01 ）, and APD of paired myocytes was increased more than single myocytes （ 181 ± 64 ms vs 28 ± 13 ms, P 〈 0. 01 ）.Conclusion： Gap junction may counteract the prolongation of APD induced by sotalol.

关 键 词：心血管病学 缝隙连接 动作电位时程 双细胞 穿孔膜片技术 

分 类 号：R331.38[医药卫生—人体生理学]