沿海甜菜的遗传转化和转基因耐盐植株的获得  

Genetic transformation of Beta maritima L. by Agrobacterium tumefaciens and production of salt tolerant transgenic plants

作　　者：张红[1] 段晓光[1] 杨爱芳[1] 张举仁[1] 

机构地区：[1]山东大学生命科学学院,济南,250100

出　　处：《高技术通讯》2005年第8期72-77,共6页Chinese High Technology Letters

基　　金：国家高技术研究发展计划(863计划)，山东省优秀中青年科学家科研奖励基金

摘　　要：以沿海甜菜丛生芽芽尖为转化受体,采用农杆菌介导法将来自大肠杆菌的编码胆碱脱氢酶的betA基因和来自拟南芥的突变的als基因转入受体细胞,通过除草剂抗性筛选、耐盐性测定和外源基因的分子检测,获得了转基因耐盐植株.PCR阳性的抗除草剂的转化小芽在分别含有1.5%、2.0%、2.5%和3.0%NaCl的培养基上进行耐盐性检测,其存活率和生物量大幅度高于未转基因的对照小芽.将移栽成活的转基因植株用盐水浇灌,转基因植株表现出比对照明显提高的耐盐性.Introduction of betA gene encoding choline dehydrogenase from E. coli and mutant als gene from Arabidopsis thaliana into the bud tips from shoots cluster of Beta maritima L. were accomplished via Agrobacterium tumefaciens-mediated transformation. The transgenic plants with salt tolerance were produced by the selection of herbicide Lvhuanglong, examination of salt tolerance and assay of PCR and PCR-Southern blotting. On the media supplemented with 1.5%, 2.0%, 2.5% or 3.0% NaCl, PCR-positive buds of herbicide tolerance showed much higher survival percentage and biomass than controls. When the plants transplanted into pots were irrigated with salt solution, transgenic plants showed obviously improved salt tolerance.

关 键 词：沿海甜菜 BETA OLS 遗传转化 转基因植株 耐盐性 甜菜 沿海 农杆菌介导法 分子检测 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]