肿瘤坏死因子-α(TNF-α)对口腔黏膜下纤维化成纤维细胞生物学效应研究  

Study for biological effect of tumor necrosis factor- αon oral fibroblasts from patients with oral submucous fibrosis

作　　者：吴颖芳[1] 方厂云[1] 阙国英[1] 

机构地区：[1]中南大学湘雅医院口腔系,湖南长沙410008

出　　处：《中国医学工程》2005年第4期363-365,共3页China Medical Engineering

基　　金：湖南省科委发展基金资助(99ssy2002-14)

摘　　要：目的阐明肿瘤坏死因子-α(TNF-α)对正常人颊黏膜成纤维细胞(NM-FB)及口腔黏膜下纤维化(OSF)患者颊黏膜成纤维细胞(OSF-FB)的生长增殖及胶原合成的影响。方法取6例OSF患者及5例正常人颊黏膜组织行成纤维细胞原代培养并传代,采用MTT法及羟脯氨酸试剂盒分别测定TNF-α处理后NM-FB及OSF-FB的生长增殖及羟脯氨酸合成情况,并在电镜下观察NM-FB及OSF-FB超微结构的改变。结果OSF-FB羟脯氨酸合成量明显大于NM-FB(P<0.01),浓度为100u/mL、1000u/mL及10000u/mL的TNF-α均能有效刺激NM-FB及OSF-FB的增殖及羟脯氨酸合成(P<0.05),电镜下亦可观察到TNF-α作用后,细胞的粗面内质网、游离核糖体明显增多,粗面内质网池扩张,线粒体旺盛。结论TNF-α有可能通过促进NM-FB及OSF-FB的增殖及胶原合成,启动OSF发生并推动其发展。[Objective] To analyze the effects of tumor necrosis factor-α on the progression of oral submucous fibrosis. [Method] Fibroblast cultures were estabhshed from the buccal mucosa of five normal persons and six patients with OSF. The study focused on the effects of tumor necrosis factor-α （TNF-α） on the growth and collagen biosynthesis of fibroblasts in vitro. And the effects of the cytokine on the ultrastructure of the fibroblasts were observed by electronmicroscopy. [Result] The study showed that TNF-α could increase the growth and the collagen synthesis of oral mucosal-derived fibroblasts. Furthermore, the ultrastructure of the fibroblasts were changed correspondingly by TNF-αtreatment. [Conclusion] The investigation confirm that TNF-α is a cytokine with upregulation effects on OSF progression.

关 键 词：肿瘤坏死因子-Α 口腔黏膜下纤维化 成纤维细胞 

分 类 号：R696[医药卫生—泌尿科学]