金菊双花总黄酮提取与纯化工艺研究  

Study on the Extraction and Purification for Total Flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel.

作　　者：杨俊 张晓静 田丽娟 王兴旺 

机构地区：[1]金陵药业股份有限公司,南京210009

出　　处：《中药材》2005年第8期703-705,共3页Journal of Chinese Medicinal Materials

摘　　要：目的筛选金菊双花总黄酮的最佳提取与纯化工艺。方法采用多种提取工艺比较、L9(34)正交实验及D101大孔吸附树脂纯化技术。结果其最佳提取工艺为8倍量55%乙醇、100℃水浴、每次1h回流提取2次,再结合D101大孔吸附树脂纯化,水、30%乙醇、70%乙醇梯度洗脱,收集70%乙醇洗脱部分浓缩干燥并测定黄酮含量为62.7%。结论此工艺简便可行,符合工业化生产要求。Objective： To optimize the extraction and purifieation conditions for total flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel, Methods： Conlparision of several extraction processes,the L9 （3^4） orthogonal design and macroreticular absorption resin D101 purification technique were performed. Results： The optimum extraction conditions were 8 volume of 55% ethanol, 1h refluence at 100℃, water bath for 2 times. The extracted fluid was absorbed with the resin D101. The elutions were water ,30% enthanol,70% enthanol. The obtained elution of 70% enthanol was evaporated and the content of total flavonoids was 62. 7%, Conclusion： The method is simple, approved and fit for industrial production.

关 键 词：金银花 菊花 总黄酮 提取 纯化 最佳提取工艺 纯化工艺 工艺研究 D101大孔吸附树脂 双花 

分 类 号：R282.71[医药卫生—中药学] R284.2[医药卫生—中医学]