MMP-2、TIMP-1在小鼠膜性肾病肾脏中的表达及意义  

Expression of TIMP-1, MMP-2 protein in kidney of mice with membranous nephropathy

作　　者：曹灵[1] 孙兴旺[2] 马跃荣[2] 刘俊[2] 王有模[2] 陈明[1] 

机构地区：[1]泸州医学院附属医院肾脏病内科,四川泸州464000 [2]泸州医学院病理学教研室,四川泸州464000

出　　处：《中国现代医学杂志》2005年第16期2421-2425,共5页China Journal of Modern Medicine

基　　金：泸州医学院科研基金支助课题(NO.04017)

摘　　要：目的探讨肾脏MMP-2、TIMP-1的表达与膜性肾病发病机制间的关系。方法把小鼠随机分为膜性肾病模型组和对照组,用免疫组织化学(SABC法)检测肾脏组织中MMP-2和TIMP-1的表达;用图像分析系统检测基底膜厚度。结果模型组和对照组肾脏中均有MMP-2、TIMP-1表达,且主要表达在肾小管上皮细胞胞浆中。模型组肾组织中MMP-2及TIMP-1表达明显强于对照组(P<0.01);模型组中MMP-2和TIMP-1表达与GBM厚度呈正相关(0.01<P<0.05),对照组MMP-2和TIMP-1表达与GBM无相关性(P>0.05)。结论在MN过程中,肾脏中MMP-2和TIMP-1参与了GBM增厚形成的机制。[Objective] To research the relativity between mechanism of MN and the expression of MMP-2, TIMP-1 proteins. [Methods] Membranous nephropathy mice animals model were used, and the MMP-2, TIMP-1 proteins expression in kidney was assessed by immunohistochemistry and the thicknesses of GBM and TBM were measured. Then the statistical methods were used to compare and analyse the data of MN group and control group. [Results] MMP-2 and TIMP-1 proteins were expressed mainly in renal tubule of MN group and control group. The level of MMP-2 and TIMP-1 proteins in MN group increased remarkably comparing with control group. In MN group, the expressions of MMP-2 and TIMP-1 protein paralleled with the thickness of GBM, and In control group, the expression of MMP-2 and TIMP-1 protein were not relative to the thickness of GBM. [Conclusion] In MN the abnormal expressions of MMP-2 and TIMP-1 protein were relative to the mechanism of MN, and TIMP-1 maybe act an important action during the developing of MN.

关 键 词：膜性肾病(MN) ECM MMP-2 TIMP-1 小鼠动物模型 

分 类 号：R692[医药卫生—泌尿科学]