牛血清白蛋白与两种金属配合物的作用研究  

Interaction of Bovine Serum Albumin with Twain Metal Complexes

作　　者：刘海萍[1] 王兴明[1] 戴亚堂[1] 宋绵新[1] 

机构地区：[1]西南科技大学材料科学与工程学院应用化学研究所,绵阳621010

出　　处：《无机化学学报》2005年第9期1422-1426,共5页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.40072020);四川省教育厅自然科学基金重点资助项目(No.2000-A56)

摘　　要：The interaction of Methylthymol Blue(MTB)-Zinc! compound and Alizarin Red S(ARS)-Aluminum" compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn!-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn! complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn! with BSA was ε=2.20×104 L·mol-1·cm-1. Conditional proportion were defined, nMTB∶nZn!∶nBSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, nARS∶nAl"∶ nBSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al" with BSA was K=8.80×108. The apparent mo- lar absorptivity of ε=2.65×104 L·mol-·1cm-1. It is suggested that combination between BSA and MTB-Zn" is due to coordination force. That combination between BSA and ARS-Al" is due to the coordination bond and electro- static force.The interaction of Methyhhymol Blue（MTB）-Zinc（Ⅱ） compound and Alizarin Red S（ARS）-Aluminum（Ⅲ） compound with Bovine serum albumin （BSA） was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn（Ⅱ）-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn（Ⅱ） complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained： the apparent molar absorptivity of MTB-Zn（Ⅱ） with BSA was ε=2.20×10^4 L·mol^-1·cm^-1. Conditional proportion were defined, nMTB：nZr（Ⅱ）：nBSA=2：2：1; condition combination constant, K=2.07×10^10. Combination proportion were defined, nARS：nAl（Ⅲ）：nBSA=6：4：1. Condition equilibrium constant of reaction of ARS-Al（Ⅲ） with BSA was K=8.80×10^8. The apparent too- lar absorptivity to coordination static force of ε=2.65×10^4 L·mol^-1·cm^-1 It is suggested that combination between BSA and MTB-Zn（Ⅲ） is due force. That combination between BSA and ARS-Al（Ⅲ） is due to the coordination bond and electrostatic force.

关 键 词：UV-Vis光谱法 甲基百里酚蓝-Zn(Ⅱ)-配合物 牛血清白蛋白 茜素红S-Al(Ⅲ)-配合物 

分 类 号：O614.124.1[理学—无机化学] O614.131[理学—化学]